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Infection and Immunity, September 2006, p. 5408-5413, Vol. 74, No. 9
0019-9567/06/$08.00+0     doi:10.1128/IAI.01840-05
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Nucleotide Sequence Analysis of the Enteropathogenic Escherichia coli Adherence Factor Plasmid pMAR7{ddagger}

Carl Brinkley ,1,{dagger},§ Valerie Burland,2,{dagger} Rogéria Keller,1 Debra J. Rose,2 Adam T. Boutin,2,|| Sara A. Klink,2,# Frederick R. Blattner,2 and James B. Kaper1*

Department of Microbiology and Immunology, Center for Vaccine Development, University of Maryland School of Medicine, Baltimore, Maryland 21201,1 Laboratory of Genetics and Genome Center, University of Wisconsin, Madison, Wisconsin 537062

Received 10 November 2005/ Returned for modification 4 January 2006/ Accepted 19 June 2006

The complete nucleotide sequence was determined for pMAR7, an enteropathogenic Escherichia coli (EPEC) adherence factor (EAF) plasmid that contains genes encoding a type IV attachment pilus (Bfp) and the global virulence regulator per. Prototypic EAF plasmid pMAR7 is self-transmissible, unlike the smaller EAF plasmid pB171, which has no genes encoding conjugative functions. The tra locus, a highly conserved 33-kb segment found in pMAR7, is similar to the tra (conjugation) region of the F plasmid. ISEc13 copies flanking the pMAR7 tra region could potentially mobilize or delete the tra genes. Hybridization of 134 EPEC strains showed that a complete tra region is present only in strains of the EPEC1 clonal group. This study confirms EPEC's potential for dissemination of virulence attributes by horizontal transfer of the EAF plasmid.

* Corresponding author. Mailing address: University of Maryland School of Medicine, Center for Vaccine Development, 685 West Baltimore St., Baltimore, MD 21201. Phone: (410) 706-2344. Fax: (410) 706-0182. E-mail: jkaper{at}umaryland.edu.

{ddagger} Supplemental material for this article may be found at http://iai.asm.org/.

Editor: J. B. Bliska

{dagger} C.B. and V.B. contributed equally to this study.

§ Present address: Department of Enteric Infections, Division of Communicable Diseases and Immunology, Walter Reed Army Institute of Research, Silver Spring, MD 20910.

|| Present address: Molecular Biology Section, Division of Biological Sciences, University of California, San Diego, CA 92093.

# Present address: Promega Corp., 2800 Woods Hollow Road, Madison, WI 53711.

Infection and Immunity, September 2006, p. 5408-5413, Vol. 74, No. 9
0019-9567/06/$08.00+0     doi:10.1128/IAI.01840-05
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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