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Infection and Immunity, January 2007, p. 146-151, Vol. 75, No. 1
0019-9567/07/$08.00+0     doi:10.1128/IAI.01011-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Role of Toll-Like Receptor 9 in Legionella pneumophila-Induced Interleukin-12 p40 Production in Bone Marrow-Derived Dendritic Cells and Macrophages from Permissive and Nonpermissive Mice{triangledown}

Cathy A. Newton,1,{dagger} Izabella Perkins,1,{dagger} Raymond H. Widen,1,2 Herman Friedman,1 and Thomas W. Klein1*

Department of Molecular Medicine, University of South Florida College of Medicine, Tampa, Florida,1 Tampa General Hospital, Tampa, Florida2

Received 27 June 2006/ Returned for modification 28 July 2006/ Accepted 12 October 2006

The progression of Legionella pneumophila infection in macrophages is controlled by the Lgn1 gene locus, which expresses the nonpermissive phenotype in cells from BALB/c mice but the permissive phenotype in cells from A/J mice. Activation of dendritic cells and macrophages by L. pneumophila is mediated by the pathogen recognition receptor Toll-like receptor 2 (TLR2); furthermore, Legionella induces innate and adaptive immune cytokines by the MyD88-dependent pathway. TLR9 is coupled to MyD88 and mediates the production of interleukin-12 (IL-12) in dendritic cells infected with other facultatively intracellular pathogens. In the current study, L. pneumophila growth in dendritic cells from BALB/c and A/J mice was examined along with the role of TLR9 in the induction of IL-12 in these cells. Dendritic cells from both strains were nonpermissive for L. pneumophila intracellular growth, suggesting that the products of the Lgn1 gene locus that control intracellular growth in macrophages do not control the growth of Legionella in dendritic cells. In addition, chloroquine treatment suppressed IL-12 p40 production in response to Legionella treatment in dendritic cells and macrophages from BALB/c and A/J mice. Furthermore, the TLR9 inhibitor ODN2088 suppressed the Legionella-induced IL-12 production in dendritic cells from both mouse strains. These results suggest that L. pneumophila is similar to other intracellular bacteria in that it stimulates the production of immune-transitioning cytokines, such as IL-12, through activation of TLR9 and that this receptor provides a common mechanism for sensing these types of microbes and inducing innate and adaptive immunity.

* Corresponding author. Mailing address: Department of Molecular Medicine, MDC Box 10, University of South Florida College of Medicine, 12901 Bruce B. Downs Boulevard, Tampa, FL 33612. Phone: (813) 974-2502. Fax: (813) 974-4151. E-mail: tklein{at}health.usf.edu.

{triangledown} Published ahead of print on 23 October 2006.

Editor: R. P. Morrison

{dagger} C.A.N. and I.P. contributed equally to this work.

Infection and Immunity, January 2007, p. 146-151, Vol. 75, No. 1
0019-9567/07/$08.00+0     doi:10.1128/IAI.01011-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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