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Infection and Immunity, January 2007, p. 334-341, Vol. 75, No. 1
0019-9567/07/$08.00+0     doi:10.1128/IAI.01063-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Resistance of Primary Murine CD4+ T Cells to Helicobacter pylori Vacuolating Cytotoxin{triangledown}

Holly M. Scott Algood,1 Victor J. Torres,2 Derya Unutmaz,2,{dagger} and Timothy L. Cover1,2,3*

Departments of Medicine,1 Microbiology and Immunology, Vanderbilt University School of Medicine,2 Veterans Affairs Tennessee Valley Healthcare System, Nashville, Tennessee3

Received 6 July 2006/ Returned for modification 14 August 2006/ Accepted 16 October 2006

Persistent colonization of the human stomach by Helicobacter pylori is a risk factor for the development of gastric cancer and peptic ulcer disease. H. pylori secretes a toxin, VacA, that targets human gastric epithelial cells and T lymphocytes and enhances the ability of H. pylori to colonize the stomach in a mouse model. To examine how VacA contributes to H. pylori colonization of the mouse stomach, we investigated whether murine T lymphocytes were susceptible to VacA activity. VacA inhibited interleukin-2 (IL-2) production by a murine T-cell line (LBRM-33), similar to its effects on a human T-cell line (Jurkat), but did not inhibit IL-2 production by primary murine splenocytes or CD4+ T cells. VacA inhibited activation-induced proliferation of primary human CD4+ T cells but did not inhibit the proliferation of primary murine CD4+ T cells. Flow cytometry studies indicated that the levels of VacA binding to primary murine CD4+ T cells were significantly lower than levels of VacA binding to human CD4+ T cells. This suggests that the resistance of primary murine CD4+ T cells to VacA is attributable, at least in part, to impaired VacA binding to these cells.

* Corresponding author. Mailing address: Division of Infectious Diseases, A2200 Medical Center North, Vanderbilt University School of Medicine, Nashville, TN 37232. Phone: (615) 322-2035. Fax: (615) 343-6160. E-mail: timothy.L.cover{at}vanderbilt.edu.

{triangledown} Published ahead of print on 30 October 2006.

Editor: D. L. Burns

{dagger} Present address: New York University School of Medicine, Joan and Joel Smilow Research Center, 522 First Avenue, Smilow 10th Floor, Rm. 1011, New York, NY 10016.

Infection and Immunity, January 2007, p. 334-341, Vol. 75, No. 1
0019-9567/07/$08.00+0     doi:10.1128/IAI.01063-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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