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Infection and Immunity, October 2007, p. 4710-4718, Vol. 75, No. 10
0019-9567/07/$08.00+0     doi:10.1128/IAI.00372-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Lipase 8 Affects the Pathogenesis of Candida albicans{triangledown}

Attila Gácser,1,{dagger}* Frank Stehr,2,{dagger} Cathrin Kröger,2 László Kredics,3 Wilhelm Schäfer,2 and Joshua D. Nosanchuk1

Departments of Medicine (Division of Infectious Diseases) and Microbiology/Immunology, Albert Einstein College of Medicine, Bronx, New York 10461,1 Center of Applied Molecular Biology, University of Hamburg, Ohnhorststrasse 18, D-22609 Hamburg, Germany,2 Department of Microbiology, University of Szeged, Közép fasor 52, H-6726 Szeged, Hungary3

Received 9 March 2007/ Returned for modification 29 April 2007/ Accepted 14 July 2007

The production of lipases can affect microbial fitness and virulence. We examined the role of the lipase 8 (LIP8) gene in the virulence of Candida albicans by constructing {Delta}lip8 strains by the URA-blaster disruption method. Reverse transcription-PCR experiments demonstrated the absence of LIP8 expression in the homozygous knockout mutants. Reconstituted strains and overexpression mutants were generated by introducing a LIP8 open reading frame under control of a constitutive actin promoter. Knockout mutants produced more mycelium, particularly at higher temperatures and pH ≥7. Diminished LIP8 expression resulted in reduced growth in lipid-containing media. Mutants deficient in the LIP8 gene were significantly less virulent in a murine intravenous infection model. The results clearly indicate that Lip8p is an important virulence factor of C. albicans.

* Corresponding author. Mailing address: Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461. Phone: (718) 430-02993. Fax: (718) 430-8968. E-mail: gacsera{at}gmail.com

{triangledown} Published ahead of print on 23 July 2007.

Editor: A. Camilli

{dagger} A.G. and F.S. contributed equally to this work.

Infection and Immunity, October 2007, p. 4710-4718, Vol. 75, No. 10
0019-9567/07/$08.00+0     doi:10.1128/IAI.00372-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Trofa, D., Gacser, A., Nosanchuk, J. D. (2008). Candida parapsilosis, an Emerging Fungal Pathogen. Clin. Microbiol. Rev. 21: 606-625 [Abstract] [Full Text]  
  • Chaffin, W. L. (2008). Candida albicans Cell Wall Proteins. Microbiol. Mol. Biol. Rev. 72: 495-544 [Abstract] [Full Text]  


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