This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Liautard, J.
Right arrow Articles by Liautard, J.-P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Liautard, J.
Right arrow Articles by Liautard, J.-P.

 Previous Article  |  Next Article 

Infection and Immunity, November 2007, p. 5167-5174, Vol. 75, No. 11
0019-9567/07/$08.00+0     doi:10.1128/IAI.00690-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Identification and Isolation of Brucella suis Virulence Genes Involved in Resistance to the Human Innate Immune System{triangledown}

Janny Liautard, Safia Ouahrani-Bettache, Véronique Jubier-Maurin, Virginie Lafont, Stephan Köhler, and Jean-Pierre Liautard*

CNRS-UMR 5236 and Université Montpellier 2, place Eugène Bataillon, 34000 Montpellier, France

Received 22 May 2007/ Returned for modification 21 June 2007/ Accepted 9 August 2007

Brucella strains are facultative intracellular pathogens that induce chronic diseases in humans and animals. This observation implies that Brucella subverts innate and specific immune responses of the host to develop its full virulence. Deciphering the genes involved in the subversion of the immune system is of primary importance for understanding the virulence of the bacteria, for understanding the pathogenic consequences of infection, and for designing an efficient vaccine. We have developed an in vitro system involving human macrophages infected by Brucella suis and activated syngeneic {gamma}9{delta}2 T lymphocytes. Under these conditions, multiplication of B. suis inside macrophages is only slightly reduced. To identify the genes responsible for this reduced sensitivity, we screened a library of 2,000 clones of transposon-mutated B. suis. For rapid and quantitative analysis of the multiplication of the bacteria, we describe a simple method based on Alamar blue reduction, which is compatible with screening a large library. By comparing multiplication inside macrophages alone and multiplication inside macrophages with activated {gamma}9{delta}2 T cells, we identified four genes of B. suis that were necessary to resist to the action of the {gamma}9{delta}2 T cells. The putative functions of these genes are discussed in order to propose possible explanations for understanding their exact role in the subversion of innate immunity.

* Corresponding author. Mailing address: Université Montpellier II, CC100, place Eugène Bataillon, 34000 Montpellier, France. Phone: 33 4 67 14 32 09. Fax: 33 4 67 14 33 38. E-mail: liautard{at}univ-montp2.fr

{triangledown} Published ahead of print on 20 August 2007.

Editor: D. L. Burns

Infection and Immunity, November 2007, p. 5167-5174, Vol. 75, No. 11
0019-9567/07/$08.00+0     doi:10.1128/IAI.00690-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»