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Infection and Immunity, November 2007, p. 5200-5209, Vol. 75, No. 11
0019-9567/07/$08.00+0     doi:10.1128/IAI.00954-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Presentation of Toxoplasma gondii Antigens via the Endogenous Major Histocompatibility Complex Class I Pathway in Nonprofessional and Professional Antigen-Presenting Cells{triangledown}

Florence Dzierszinski,1* Marion Pepper,2 Jason S. Stumhofer,2 David F. LaRosa,3 Emma H. Wilson,2 Laurence A. Turka,3 Sandra K. Halonen,4 Christopher A. Hunter,2 and David S. Roos1

Departments of Biology,1 Pathobiology,2 Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104,3 Department of Microbiology, Montana State University, Bozeman, Montana 597174

Received 12 July 2007/ Returned for modification 15 August 2007/ Accepted 30 August 2007

Challenge with the intracellular protozoan parasite Toxoplasma gondii induces a potent CD8+ T-cell response that is required for resistance to infection, but many questions remain about the factors that regulate the presentation of major histocompatibility complex class I (MHC-I)-restricted parasite antigens and about the role of professional and nonprofessional accessory cells. In order to address these issues, transgenic parasites expressing ovalbumin (OVA), reagents that track OVA/MHC-I presentation, and OVA-specific CD8+ T cells were exploited to compare the abilities of different infected cell types to stimulate CD8+ T cells and to define the factors that contribute to antigen processing. These studies reveal that a variety of infected cell types, including hematopoietic and nonhematopoietic cells, are capable of activating an OVA-specific CD8+ T-cell hybridoma, and that this phenomenon is dependent on the transporter associated with antigen processing and requires live T. gondii. Several experimental approaches indicate that T-cell activation is a consequence of direct presentation by infected host cells rather than cross-presentation. Surprisingly, nonprofessional antigen-presenting cells (APCs) were at least as efficient as dendritic cells at activating this MHC-I-restricted response. Studies to assess whether these cells are involved in initiation of the CD8+ T-cell response to T. gondii in vivo show that chimeric mice expressing MHC-I only in nonhematopoietic compartments are able to activate OVA-specific CD8+ T cells upon challenge. These findings associate nonprofessional APCs with the initial activation of CD8+ T cells during toxoplasmosis.

* Corresponding author. Present address: Institute of Parasitology, McGill University, Sainte-Anne-de-Bellevue, Québec H9X 3V9, Canada. Phone: (514) 398-8764. Fax: (514) 398-7857. E-mail: florence.dzierszinski{at}mcgill.ca

{triangledown} Published ahead of print on 10 September 2007.

Editor: W. A. Petri, Jr.

Infection and Immunity, November 2007, p. 5200-5209, Vol. 75, No. 11
0019-9567/07/$08.00+0     doi:10.1128/IAI.00954-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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