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Infection and Immunity, November 2007, p. 5460-5464, Vol. 75, No. 11
0019-9567/07/$08.00+0     doi:10.1128/IAI.00773-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Antibody-Independent, CD4+ T-Cell-Dependent Protection against Pneumococcal Colonization Elicited by Intranasal Immunization with Purified Pneumococcal Proteins{triangledown}

Alan Basset,1 Claudette M. Thompson,2 Susan K. Hollingshead,3 David E. Briles,3 Edwin W. Ades,4 Marc Lipsitch,2 and Richard Malley1*

Division of Infectious Diseases, Department of Medicine, Children's Hospital Boston, Boston, Massachusetts,1 Departments of Epidemiology, Immunology, and Infectious Diseases, Harvard School of Public Health, Boston, Massachusetts,2 Department of Microbiology, University of Alabama at Birmingham, Birmingham, Alabama,3 Division of Bacterial Diseases, National Center for Immunizations and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia4

Received 7 June 2007/ Returned for modification 14 July 2007/ Accepted 29 July 2007

Immunity to pneumococcal colonization in mice by exposure to live or killed pneumococci has been shown to be antibody independent but dependent on CD4+ T cells. Here we show that intranasal immunization with pneumococcal proteins (pneumococcal surface protein C, adhesin A, and a pneumolysoid) can elicit a similar mechanism of protection. Colonization could be significantly reduced in mice congenitally deficient in immunoglobulins after intranasal immunization with this mixture of proteins; conversely, the depletion of CD4+ T cells in immunized wild-type mice at the time of challenge eliminated the protection afforded by immunization. Overall, our results show that intranasal immunization with a mixture of pneumococcal proteins protects against colonization in an antibody-independent, CD4+ T-cell-dependent manner.

* Corresponding author. Mailing address: Division of Infectious Diseases, Children's Hospital Boston, Enders 861.3, 300 Longwood Avenue, Boston, MA 02115. Phone: (617) 919-2902. Fax: (617) 730-0255. E-mail: richard.malley{at}childrens.harvard.edu

{triangledown} Published ahead of print on 13 August 2007.

Editor: A. Camilli

Infection and Immunity, November 2007, p. 5460-5464, Vol. 75, No. 11
0019-9567/07/$08.00+0     doi:10.1128/IAI.00773-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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