This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Coombes, B. K.
Right arrow Articles by Finlay, B. B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Coombes, B. K.
Right arrow Articles by Finlay, B. B.

 Previous Article  |  Next Article 

Infection and Immunity, February 2007, p. 574-580, Vol. 75, No. 2
0019-9567/07/$08.00+0     doi:10.1128/IAI.00985-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

SseL Is a Salmonella-Specific Translocated Effector Integrated into the SsrB-Controlled Salmonella Pathogenicity Island 2 Type III Secretion System{triangledown}

Brian K. Coombes,1,2,{dagger}* Michael J. Lowden,3,{dagger} Jennifer L. Bishop,3 Mark E. Wickham,3 Nat F. Brown,3 Nancy Duong,1 Suzanne Osborne,1 Ohad Gal-Mor,3 and B. Brett Finlay3

Department of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, ON,1 Public Heath Agency of Canada, Laboratory for Foodborne Zoonoses, Guelph, ON,2 Michael Smith Laboratories and Department of Microbiology and Immunology, University of British Columbia, Vancouver, BC, Canada3

Received 21 June 2006/ Returned for modification 21 August 2006/ Accepted 29 November 2006

Bacterial pathogens use horizontal gene transfer to acquire virulence factors that influence host colonization, alter virulence traits, and ultimately shape the outcome of disease following infection. One hallmark of the host-pathogen interaction is the prokaryotic type III secretion system that translocates virulence factors into host cells during infection. Salmonella enterica possesses two type III secretion systems that are utilized during host colonization and intracellular replication. Salmonella pathogenicity island 2 (SPI2) is a genomic island containing approximately 30 contiguous genes required to assemble a functional secretion system including the two-component regulatory system called SsrA-SsrB that positively regulates transcription of the secretion apparatus. We used transcriptional profiling with DNA microarrays to search for genes that coregulate with the SPI2 type III secretion machinery in an SsrB-dependent manner. Here we report the identification of a Salmonella-specific translocated effector called SseL that is required for full virulence during murine typhoid-like disease. Analysis of infected macrophages using fluorescence-activated cell sorting revealed that sseL is induced inside cells and requires SsrB for expression. SseL is retained predominantly in the cytoplasm of infected cells following translocation by the type III system encoded in SPI2. Animal infection experiments with sseL mutant bacteria indicate that integration of SseL into the SsrB response regulatory system contributes to systemic virulence of this pathogen.

* Corresponding author. Mailing address: Department of Biochemistry and Biomedical Sciences, McMaster University, Health Sciences Centre, Room 4H17, 1200 Main St. West, Hamilton, Ontario L8N 3Z5, Canada. Phone: (905) 525-9140, ext. 22159. Fax: (905) 522-9033. E-mail: coombes{at}mcmaster.ca.

{triangledown} Published ahead of print on 11 December 2006.

Editor: V. J. DiRita

{dagger} These authors contributed equally to this work.

Infection and Immunity, February 2007, p. 574-580, Vol. 75, No. 2
0019-9567/07/$08.00+0     doi:10.1128/IAI.00985-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Le Negrate, G., Faustin, B., Welsh, K., Loeffler, M., Krajewska, M., Hasegawa, P., Mukherjee, S., Orth, K., Krajewski, S., Godzik, A., Guiney, D. G., Reed, J. C. (2008). Salmonella Secreted Factor L Deubiquitinase of Salmonella typhimurium Inhibits NF-{kappa}B, Suppresses I{kappa}B{alpha} Ubiquitination and Modulates Innate Immune Responses. J. Immunol. 180: 5045-5056 [Abstract] [Full Text]  
  • Duong, N., Osborne, S., Bustamante, V. H., Tomljenovic, A. M., Puente, J. L., Coombes, B. K. (2007). Thermosensing Coordinates a Cis-regulatory Module for Transcriptional Activation of the Intracellular Virulence System in Salmonella enterica Serovar Typhimurium. J. Biol. Chem. 282: 34077-34084 [Abstract] [Full Text]  
  • Thijs, I. M. V., De Keersmaecker, S. C. J., Fadda, A., Engelen, K., Zhao, H., McClelland, M., Marchal, K., Vanderleyden, J. (2007). Delineation of the Salmonella enterica Serovar Typhimurium HilA Regulon through Genome-Wide Location and Transcript Analysis. J. Bacteriol. 189: 4587-4596 [Abstract] [Full Text]  
  • Rytkonen, A., Poh, J., Garmendia, J., Boyle, C., Thompson, A., Liu, M., Freemont, P., Hinton, J. C. D., Holden, D. W. (2007). SseL, a Salmonella deubiquitinase required for macrophage killing and virulence. Proc. Natl. Acad. Sci. USA 104: 3502-3507 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»