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Infection and Immunity, February 2007, p. 760-765, Vol. 75, No. 2
0019-9567/07/$08.00+0     doi:10.1128/IAI.01594-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Comparative Analysis of the Abilities of Shiga Toxins 1 and 2 To Bind to and Influence Neutrophil Apoptosis{triangledown}

Michael J. Flagler,1 Jane E. Strasser,2 Claudia L. Chalk,2 and Alison A. Weiss1*

Department of Molecular Genetics, Biochemistry, and Microbiology, University of Cincinnati, Cincinnati, Ohio 45267,1 Division of Infectious Diseases, Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio 452292

Received 2 October 2006/ Returned for modification 20 October 2006/ Accepted 6 November 2006

Hemolytic-uremic syndrome (HUS), the life-threatening complication following infection by the intestinal pathogen Escherichia coli O157:H7, is due to the ability of the pathogen to produce toxins in the Shiga toxin (Stx) family. Activated neutrophils are observed in HUS patients, yet it is unclear whether Stx exerts a direct effect on neutrophils or whether the toxin acts indirectly. The effect of Stx1 and Stx2 on human neutrophils was examined. Neither Stx1 nor Stx2 altered the rate of neutrophil apoptosis. Minimal binding of either toxin to neutrophils was observed, and the toxin was easily eluted from the cells. Stx1 and Stx2 were found to circulate in the plasma of mice following intravenous injection, and both toxins were cleared rapidly from the blood. Together these results suggest that neither Stx1 nor Stx2 interacts directly with neutrophils.

* Corresponding author. Mailing address: Department of Molecular Genetics, Biochemistry, and Microbiology, University of Cincinnati, 231 Albert Sabin Way, Cincinnati, OH 45267-0524. Phone: (513) 558-2820. Fax: (513) 558-8474. E-mail: Alison.Weiss{at}uc.edu.

{triangledown} Published ahead of print on 13 November 2006.

Editor: A. D. O'Brien

Infection and Immunity, February 2007, p. 760-765, Vol. 75, No. 2
0019-9567/07/$08.00+0     doi:10.1128/IAI.01594-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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