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Therapeutic Chlamydophila abortus and C. pecorum Vaccination Transiently Reduces Bovine Mastitis Associated with Chlamydophila Infection

Bayer HealthCare AG., Osterather Strasse 1a, D-50739 Köln, Germany,¹ Department of Pathobiology, College of Veterinary Medicine, Auburn University, Auburn, Alabama 36849,² Friedrich-Loeffler-Institut (Federal Research Institute for Animal Health), Naumburger Str. 96a, D-07743 Jena, Germany³

Received 28 April 2006/ Returned for modification 2 June 2006/ Accepted 13 November 2006

Infections with Chlamydophila abortus and C. pecorum are highly prevalent in cattle and have been associated with bovine mastitis. A prospective cohort study was conducted with a herd of 140 Holstein dairy cows to investigate the influence of Chlamydophila infection on subclinical inflammation of the bovine mammary gland as characterized by somatic cell numbers in milk. PCR detection of C. abortus and low serum antibody levels against Chlamydophila spp. were significantly associated with subclinical mastitis. To examine the effect of the infection by response modification, immune perturbation was done by two subcutaneous administrations of an experimental vaccine preparation of inactivated C. abortus and C. pecorum elementary bodies. Vaccination against Chlamydophila highly significantly decreased milk somatic cell numbers, thus reducing bovine mastitis, and increased antibody levels against Chlamydophila but did not eliminate shedding of C. abortus in milk as detected by PCR. The protective effect peaked at 11 weeks after vaccination and lasted for a total of 14 weeks. Vaccination with the Chlamydophila vaccine, a mock vaccine, or a combination vaccine against bovine viral diseases highly significantly increased C. abortus shedding in milk for 1 week, presumably mediated by the vaccine adjuvant. In summary, this study shows an etiological involvement of the widespread Chlamydophila infections in bovine mastitis, a herd disease of critical importance for the dairy industry. Furthermore, this investigation shows the potential for temporary improvement of chlamydial disease by therapeutic vaccination. Chlamydophila vaccination of cattle might serve as a testing ground for vaccines against human chlamydial infections.

Published ahead of print on 21 November 2006.

Editor: D. L. Burns