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Infection and Immunity, February 2007, p. 932-940, Vol. 75, No. 2
0019-9567/07/$08.00+0     doi:10.1128/IAI.00736-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Reduced Phase Switch Capacity and Functional Adhesin Expression of Type 1-Fimbriated Escherichia coli from Immunoglobulin A-Deficient Individuals{triangledown}

Forough L. Nowrouzian,1* Vanda Friman,2 Ingegerd Adlerberth,1 and Agnes E. Wold1

Department of Clinical Bacteriology,1 Department of Infectious Diseases, Göteborg University, Göteborg, Sweden2

Received 8 May 2006/ Returned for modification 12 September 2006/ Accepted 2 November 2006

The mannose-specific adhesin of type 1 fimbriae is the most common adhesin in Escherichia coli. One receptor for this adhesin is the carbohydrate chains of secretory immunoglobulin A (S-IgA), and intestinal E. coli from IgA-deficient individuals has a reduced capacity to adhere to mannose-containing receptors. Here, we investigated the expression of the mannose-specific adhesin and its capacity to switch to the fimbriated phenotype in colonic resident and transient E. coli strains isolated from control (n = 16) and IgA-deficient (n = 17) persons. Resident E. coli strains from IgA-deficient individuals displayed weaker mannose-specific adherence to colonic cells than resident strains from control individuals (21 versus 44 bacteria/cell, P = 0.0009) due to three mechanisms: a lower carriage rate of the fimH gene (90% versus 97%, not significant), more frequent failure to switch on the fim genes (30% versus 6%, P = 0.02), and the reduced adhesive potential of fimH+ isolates capable of phase switch (26 versus 46 bacteria/cell, P = 0.02). On the other hand, resident strains from IgA-deficient individuals displayed stronger mannose-resistant adherence than resident strains from control individuals (P = 0.04) and transient strains from IgA-deficient individuals (P = 0.01). The presence of S-IgA appears to favor the establishment of E. coli clones which readily express mannose-specific adhesins in the bowel microbiota.

* Corresponding author. Mailing address: Department of Clinical Bacteriology, Guldhedsgatan 10, S-413 46 Göteborg, Sweden. Phone: 46-31-3424887. Fax: 46-31-3424975. E-mail: forough.nowrouzian{at}microbio.gu.se.

{triangledown} Published ahead of print on 13 November 2006.

Editor: V. J. DiRita

Infection and Immunity, February 2007, p. 932-940, Vol. 75, No. 2
0019-9567/07/$08.00+0     doi:10.1128/IAI.00736-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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