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Infection and Immunity, February 2007, p. 988-996, Vol. 75, No. 2
0019-9567/07/$08.00+0     doi:10.1128/IAI.00948-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Protective Effect of the Nramp1 BB Genotype against Brucella abortus in the Water Buffalo (Bubalus bubalis){triangledown}

Rosanna Capparelli,1 Flora Alfano,2 Maria Grazia Amoroso,3 Giorgia Borriello,3 Domenico Fenizia,2 Antonio Bianco,4 Sante Roperto,5 Franco Roperto,5 and Domenico Iannelli3*

Faculty of Biotechnological Sciences, University of Naples Federico II, Naples, Italy,1 Istituto Zooprofilattico Sperimentale per il Mezzogiorno, Portici, Italy,2 Chair of Immunology, Faculty of Agriculture, University of Naples Federico II, Naples, Italy,3 Regione Campania, Assessorato all'Agricoltura, Naples, Italy,4 Faculty of Veterinary Medicine, University of Naples Federico II, Naples, Italy5

Received 14 June 2006/ Returned for modification 9 August 2006/ Accepted 20 November 2006

We tested 413 water buffalo cows (142 cases and 271 controls) for the presence of anti-Brucella abortus antibodies (by the skin test, the agglutination test, and the complement fixation test) and the Nramp1 genotype (by capillary electrophoresis). Four alleles (Nramp1A, -B, -C, and -D) were detected in the 3' untranslated region of the Nramp1 gene. The BB genotype was represented among only controls, providing evidence that this genotype confers resistance to Brucella abortus. The monocytes from the BB (resistant) subjects displayed a higher basal level of Nramp1 mRNA and a lower number of viable intracellular bacteria than did the monocytes from AA (susceptible) subjects. The higher basal level of the antibacterial protein Nramp1 most probably provides the BB animals with the possibility of controlling bacteria immediately after their entry inside the cell.

* Corresponding author. Mailing address: Cattedra di Immunologia, Dipartimento di Scienze Zootecniche e Ispezione degli Alimenti, Università degli Studi di Napoli Federico II, Via Università, 133, 80055, Portici, Naples, Italy. Phone: 39 081 2539277. Fax: 39 081 7762886. E-mail: iannelli{at}unina.it.

{triangledown} Published ahead of print on 4 December 2006.

Editor: V. J. DiRita

Infection and Immunity, February 2007, p. 988-996, Vol. 75, No. 2
0019-9567/07/$08.00+0     doi:10.1128/IAI.00948-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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