This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hogan, L. H.
Right arrow Articles by Sandor, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hogan, L. H.
Right arrow Articles by Sandor, M.

 Previous Article  |  Next Article 

Infection and Immunity, March 2007, p. 1154-1166, Vol. 75, No. 3
0019-9567/07/$08.00+0     doi:10.1128/IAI.00943-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Virally Activated CD8 T Cells Home to Mycobacterium bovis BCG-Induced Granulomas but Enhance Antimycobacterial Protection Only in Immunodeficient Mice{triangledown}

Laura H. Hogan,1 Dominic O. Co,1,{dagger} Jozsef Karman,1,{ddagger} Erika Heninger,1 M. Suresh,2 and Matyas Sandor1*

Department of Pathology and Laboratory Medicine, University of Wisconsin School of Medicine and Public Health, Madison, Wisconsin 53706,1 Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin, Madison, Wisconsin 537062

Received 13 June 2006/ Returned for modification 9 August 2006/ Accepted 10 December 2006

The effect of secondary infections on CD4 T-cell-regulated chronic granulomatous inflammation is not well understood. Here, we have investigated the effect of an acute viral infection on the cellular composition and bacterial protection in Mycobacterium bovis strain bacille Calmette-Guérin (BCG)-induced granulomas using an immunocompetent and a partially immunodeficient murine model. Acute lymphocytic choriomeningitis virus (LCMV) coinfection of C57BL/6 mice led to substantial accumulation of gamma interferon (IFN-{gamma})-producing LCMV-specific T cells in liver granulomas and increased local IFN-{gamma}. Despite traffic of activated T cells that resulted in a CD8 T-cell-dominated granuloma, the BCG liver organ load was unaltered from control levels. In OT-1 T-cell-receptor (TCR) transgenic mice, ovalbumin (OVA) immunization or LCMV coinfection of BCG-infected mice induced CD8 T-cell-dominated granulomas containing large numbers of non-BCG-specific activated T cells. The higher baseline BCG organ load in this CD8 TCR transgenic animal allowed us to demonstrate that OVA immunization and LCMV coinfection increased anti-BCG protection. The bacterial load remained substantially higher than in mice with a more complete TCR repertoire. Overall, the present study suggests that peripherally activated CD8 T cells can be recruited to chronic inflammatory sites, but their contribution to protective immunity is limited to conditions of underlying immunodeficiency.

* Corresponding author. Mailing address: Rm. 5468 MSC, Department of Pathology and Laboratory Medicine, 1300 University Ave., Madison, WI 53706. Phone: (608)265-8715. Fax: (608)262-0846. E-mail: msandor{at}wisc.edu.

{triangledown} Published ahead of print on 18 December 2006.

Editor: J. L. Flynn

{dagger} Present address: Children's Hospital of Wisconsin, Milwaukee, WI.

{ddagger} Present address: Center for Neurologic Diseases, Brigham and Women's Hospital, 77 Avenue Louis Pasteur, HIM 785, Boston, MA 02115-5817.

Infection and Immunity, March 2007, p. 1154-1166, Vol. 75, No. 3
0019-9567/07/$08.00+0     doi:10.1128/IAI.00943-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Lee, J., Reinke, E. K., Zozulya, A. L., Sandor, M., Fabry, Z. (2008). Mycobacterium bovis Bacille Calmette-Guerin Infection in the CNS Suppresses Experimental Autoimmune Encephalomyelitis and Th17 Responses in an IFN-{gamma}-Independent Manner. J. Immunol. 181: 6201-6212 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»