This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Marks, M.
Right arrow Articles by Pirofski, L.-a.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Marks, M.
Right arrow Articles by Pirofski, L.-a.

 Previous Article  |  Next Article 

Infection and Immunity, April 2007, p. 1586-1597, Vol. 75, No. 4
0019-9567/07/$08.00+0     doi:10.1128/IAI.01579-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Influence of Neutropenia on the Course of Serotype 8 Pneumococcal Pneumonia in Mice{triangledown}

Matthew Marks,1,{dagger} Tamika Burns,1,{dagger} Maria Abadi,3 Beza Seyoum,2 Justin Thornton,4 Elaine Tuomanen,4 and Liise-anne Pirofski1,2*

Division of Infectious Diseases, Montefiore Medical Center and Albert Einstein College of Medicine, Bronx, New York,1 Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, New York,2 Department of Pathology, Albert Einstein College of Medicine and Jacobi Medical Center, Bronx, New York,3 Department of Infectious Diseases, St. Jude Children's Research Hospital, Memphis, Tennessee4

Received 29 September 2006/ Returned for modification 6 December 2006/ Accepted 18 January 2007

Polymorphoneutrophils (PMNs) are important effector cells in host defense against pneumonia. However, PMNs can also induce inflammation and tissue damage. To investigate the contribution of PMNs to host defense against pneumococcal pneumonia, we determined the effect of the PMN-depleting rat monoclonal antibody RB6-8C5 (RB6) on survival and inflammatory and cellular response in the lungs to a lethal intranasal infection with a serotype 8 pneumococcus in BALB/c mice. Control mice received rat immunoglobulin G (rIgG). Strikingly, the survival of RB6-treated mice was significantly prolonged compared to that of rIgG-treated mice. Although the numbers of CFU in the lungs were statistically similar in both groups 4, 24, and 32 h after infection, rIgG-treated mice developed higher levels of bacteremia, and histopathological examination of the lungs of infected mice revealed marked differences between RB6- and rIgG-treated mice. RB6-treated mice had focal, perivascular lesions without accompanying parenchymal inflammation, and rIgG-treated mice had diffuse, interstitial parenchymal inflammation. Lung homogenates from the rIgG-treated mice had more leukocytes and significantly more total and apoptotic PMNs as determined by fluorescence-activated cell sorter analysis with Annexin V and terminal deoxynucleotidyltransferase-mediated dUTP-biotin nick end labeling staining of lung tissue samples. Studies with a pneumolysin-deficient mutant of the serotype 8 strain we used also demonstrated the prolonged survival of RB6- compared to rIgG-treated mice. Taken together, our findings suggest that PMNs enhance the likelihood of early death and alter the pathological response to pneumococcal lung infection in BALB/c mice with serotype 8 pneumonia without significantly affecting bacterial clearance or the cytokine response.

* Corresponding author. Mailing address: Division of Infectious Diseases, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Forchheimer Bldg, Room 709, Bronx, NY 10461. Phone: (718) 430-2372. Fax: (718) 430-2292. E-mail: Pirofski{at}aecom.yu.edu.

{triangledown} Published ahead of print on 12 February 2007.

Editor: J. N. Weiser

{dagger} M.M. and T.B. contributed equally to this work.

Infection and Immunity, April 2007, p. 1586-1597, Vol. 75, No. 4
0019-9567/07/$08.00+0     doi:10.1128/IAI.01579-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Tian, H., Weber, S., Thorkildson, P., Kozel, T. R., Pirofski, L.-a. (2009). Efficacy of Opsonic and Nonopsonic Serotype 3 Pneumococcal Capsular Polysaccharide-Specific Monoclonal Antibodies against Intranasal Challenge with Streptococcus pneumoniae in Mice. Infect. Immun. 77: 1502-1513 [Abstract] [Full Text]  
  • Leendertse, M., Willems, R. J. L., Giebelen, I. A. J., Roelofs, J. J. T. H., Bonten, M. J. M., van der Poll, T. (2009). Neutrophils Are Essential for Rapid Clearance of Enterococcus faecium in Mice. Infect. Immun. 77: 485-491 [Abstract] [Full Text]  
  • Marriott, H. M., Jackson, L. E., Wilkinson, T. S., Simpson, A. J., Mitchell, T. J., Buttle, D. J., Cross, S. S., Ince, P. G., Hellewell, P. G., Whyte, M. K. B., Dockrell, D. H. (2008). Reactive Oxygen Species Regulate Neutrophil Recruitment and Survival in Pneumococcal Pneumonia. Am. J. Respir. Crit. Care Med. 177: 887-895 [Abstract] [Full Text]  
  • van Faassen, H., KuoLee, R., Harris, G., Zhao, X., Conlan, J. W., Chen, W. (2007). Neutrophils Play an Important Role in Host Resistance to Respiratory Infection with Acinetobacter baumannii in Mice. Infect. Immun. 75: 5597-5608 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»