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Infection and Immunity, April 2007, p. 1721-1729, Vol. 75, No. 4
0019-9567/07/$08.00+0     doi:10.1128/IAI.01183-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Cloning, Expression, and Characterization of the Superantigen Streptococcal Pyrogenic Exotoxin G from Streptococcus dysgalactiae{triangledown}

Jizi Zhao,1,2,{dagger} Tomohito Hayashi,3 Susanna Saarinen,4 Anastassios C. Papageorgiou,4 Hidehito Kato,2 Ken'ichi Imanishi,2 Teruo Kirikae,1 Ryo Abe,3 Takehiko Uchiyama,2 and Tohru Miyoshi-Akiyama1*

Department of Infectious Diseases, Research Institute, International Medical Center of Japan, 1-21-1, Toyama, Shinjuku-ku, Tokyo 162-8655, Japan,1 Department of Microbiology and Immunology of Tokyo Women's Medical University, 8-1 Kawada-cho, Shinjuku-ku, Tokyo 162-8666, Japan,2 Division of Immunobiology, Research Institute for Biological Science, Tokyo University of Science, 2641, Yamasaki, Noda, Chiba 278-8510, Japan,3 Turku Centre for Biotechnology, University of Turku, Turku 20521, Finland4

Received 27 July 2006/ Returned for modification 10 August 2006/ Accepted 24 December 2006

We identified seven novel variants of streptococcal pyrogenic exotoxin G (SPEGG), a superantigen, in Streptococcus dysgalactiae subsp. dysgalactiae or equisimilis isolates from clinical cases of infection in humans and animals. Phylogenetic analysis of the SPEGG variants indicated two clades in the dendrogram: one composed of variants derived from the bacteria isolated from the humans and the other composed of variants from the bacteria isolated from the animals. Bovine peripheral blood mononuclear cells (PBMCs) were stimulated effectively by recombinant SPEGGs (rSPEGGs) expressed in Escherichia coli, while human PBMCs were not stimulated well by any of the rSPEGGs tested. SPEGGs selectively stimulated bovine T cells bearing Vß1,10 and Vß4. Bovine serum showed reactivity to the rSPEGG proteins. These results indicated that SPEGGs have properties as superantigens, and it is likely that SPEGGs play a pathogenic role in animals.

* Corresponding author. Mailing address: Department of Infectious Diseases, Research Institute, International Medical Center of Japan, 1-21-1, Toyama, Shinjuku-ku, Tokyo 162-8655, Japan. Phone: 81-3-3202-7181, ext. 2903. Fax: 81-3-3202-7364. E-mail: takiyam{at}ri.imcj.go.jp

{triangledown} Published ahead of print on 5 February 2007.

Editor: A. Camilli

{dagger} Present address: Department of Special Pathogens, International Research Center for Infectious Disease, Research Institute for Microbial Disease, Osaka University, 3-1, Yamadoka, Suita, 565-0875, Osaka, Japan.

Infection and Immunity, April 2007, p. 1721-1729, Vol. 75, No. 4
0019-9567/07/$08.00+0     doi:10.1128/IAI.01183-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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