This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental material
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Into, T.
Right arrow Articles by Matsushita, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Into, T.
Right arrow Articles by Matsushita, K.

 Previous Article  |  Next Article 

Infection and Immunity, May 2007, p. 2253-2259, Vol. 75, No. 5
0019-9567/07/$08.00+0     doi:10.1128/IAI.00141-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Synthesis and Characterization of a Dipalmitoylated Lipopeptide Derived from Paralogous Lipoproteins of Mycoplasma pneumoniae{triangledown} ,{dagger}

Takeshi Into,1,{ddagger}* Jun-ichi Dohkan,1,{ddagger} Megumi Inomata,1 Misako Nakashima,1 Ken-ichiro Shibata,2 and Kenji Matsushita1

Department of Oral Disease Research, National Institute for Longevity Sciences, National Center for Geriatrics and Gerontology, Obu, Aichi, Japan,1 Laboratory of Oral Molecular Microbiology, Department of Oral Pathobiological Science, Hokkaido University Graduate School of Dental Medicine, Sapporo, Japan2

Received 28 January 2007/ Accepted 12 February 2007

Genomic analysis of Mycoplasma pneumoniae revealed the existence of a large number of putative lipoprotein genes compared with the numbers in other bacteria. However, the pathogenic roles of M. pneumoniae lipoproteins are still obscure. In this study, we synthesized a lipopeptide (designated M. pneumoniae paralogous lipoprotein 1 [MPPL-1]) in which an S-dipalmitoylglyceryl cysteine was coupled to a peptide with a consensus sequence of a putative paralogous lipoprotein group characteristic of M. pneumoniae. The cytokine-inducing activity of MPPL-1 in human monocytic cells was much weaker (~700-fold weaker) than that of the known mycoplasmal S-dipalmitoylated lipopeptide FSL-1 or MALP-2. MPPL-1 required Toll-like receptor (TLR2) to activate NF-{kappa}B-dependent gene transcription in HEK293 cells, although a 1,000-fold-larger amount of MPPL-1 was needed to exert activity similar to that of FSL-1 in the cells. TLR2-mediated recognition of MPPL-1 was synergistically upregulated by TLR6 but not by TLR1 or TLR10, although the activity was still weak. In addition, MPPL-1 did not antagonize FSL-1 recognition in human monocytic cells and TLR2/TLR6-expressing HEK293 cells. Thus, these results suggest that there is preferential selective recognition of diacylated lipopeptides due to the magnitude of an affinity with TLR2 and TLR6 and the roles of increased paralogous lipoprotein genes of M. pneumoniae in evasion of TLR2 recognition.

* Corresponding author. Mailing address: Department of Oral Disease Research, National Institute for Longevity Sciences, National Center for Geriatrics and Gerontology, 36-3 Gengo, Morioka, Obu, Aichi 474-8522, Japan. Phone: 81-562-44-5651, ext. 5064. Fax: 81-562-46-8684. E-mail: into{at}nils.go.jp

{triangledown} Published ahead of print on 26 February 2007.

{dagger} Supplemental material for this article may be found at http://iai.asm.org/.

Editor: D. L. Burns

{ddagger} T.I. and J.D. contributed equally to this work.

Infection and Immunity, May 2007, p. 2253-2259, Vol. 75, No. 5
0019-9567/07/$08.00+0     doi:10.1128/IAI.00141-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Brant, K. A., Fabisiak, J. P. (2008). Nickel Alterations of TLR2-Dependent Chemokine Profiles in Lung Fibroblasts Are Mediated by COX-2. Am. J. Respir. Cell Mol. Bio. 38: 591-599 [Abstract] [Full Text]  
  • Into, T., Inomata, M., Nakashima, M., Shibata, K.-i., Hacker, H., Matsushita, K. (2008). Regulation of MyD88-Dependent Signaling Events by S Nitrosylation Retards Toll-Like Receptor Signal Transduction and Initiation of Acute-Phase Immune Responses. Mol. Cell. Biol. 28: 1338-1347 [Abstract] [Full Text]  
  • Shimizu, T., Kida, Y., Kuwano, K. (2008). Mycoplasma pneumoniae-Derived Lipopeptides Induce Acute Inflammatory Responses in the Lungs of Mice. Infect. Immun. 76: 270-277 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»