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Th1 and Th2 Cells Help CD8 T-Cell Responses

Melinda J. Ekkens,^1, Devon J. Shedlock,^2, EuiHye Jung,¹ Amy Troy,^2, Erika L. Pearce,^2,|| Hao Shen,¹ and Edward J. Pearce^1*

Department of Pathobiology, School of Veterinary Medicine,¹ Department of Microbiology, School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19014²

Received 18 August 2006/ Returned for modification 30 September 2006/ Accepted 12 February 2007

Help from CD4 T cells is often important for the establishment of primary and memory CD8 T-cell responses. However, it has yet to be determined whether T helper polarization affects the delivery of help and/or whether responding CD8 T cells helped by Th1 or Th2 cells express distinct effector properties. To address these issues, we compared CD8 T-cell responses in the context of Th1 or Th2 help by injecting dendritic cells copulsed with the major histocompatibility complex class I-restricted OVA peptide plus, respectively, bacterial or helminth antigens. We found that Th2 cells, like Th1 cells, can help primary and long-lived memory CD8 T-cell responses. Experiments in interleukin-12 (IL-12)^–/– and IL-4^–/– mice, in which polarized Th1 or Th2 responses, respectively, fail to develop, indicate that the underlying basis of CD4 help is independent of attributes acquired as a response to polarization.

Published ahead of print on 26 February 2007.

Editor: J. F. Urban, Jr.

Present address: Department of Biology, Columbia Union College, Takoma Park, MD.

Present address: Vaccine Research Center, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD.

Present address: Department of Pathology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA 19104.

^|| Present address: Department of Pathology and Laboratory Medicine, Abramson Family Cancer Research Institute, School of Medicine, University of Pennsylvania, Philadelphia, PA 19104.

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