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Bile Salts Stimulate Recruitment of IpaB to the Shigella flexneri Surface, Where It Colocalizes with IpaD at the Tip of the Type III Secretion Needle

Andrew J. Olive,¹ Roma Kenjale,^1, Marianela Espina,^1, David S. Moore,² Wendy L. Picking,¹ and William D. Picking^1*

Department of Molecular Biosciences,¹ KU Center for Research, Inc., University of Kansas, Lawrence, Kansas 66045²

Received 3 October 2006/ Returned for modification 22 November 2006/ Accepted 30 January 2007

Shigella flexneri uses its type III secretion apparatus (TTSA) to deliver invasins into human cells. This TTSA possesses an external needle with IpaD at its tip. We now show that deoxycholate promotes the stable recruitment of IpaB to the needle tip without inducing a rapid burst of type III secretion. The maintenance of IpaB at the needle tip requires a stable association of IpaD with the Shigella surface. This is the first demonstration of a translocator protein being stably associated with the TTSA needle.

Published ahead of print on 12 February 2007.

Editor: V. J. DiRita

Current address: Department of Pediatrics, Duke University, Durham, NC.

Current address: Infection, Immunity, Injury and Repair Program, The Hospital for Sick Children, University of Toronto, Toronto, Canada.

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