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Infection and Immunity, June 2007, p. 3062-3069, Vol. 75, No. 6
0019-9567/07/$08.00+0     doi:10.1128/IAI.00202-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

CD14 Mediates Cross Talk between Mononuclear Cells and Fibroblasts for Upregulation of Matrix Metalloproteinase 9 by Borrelia burgdorferi{triangledown}

Zhihui Zhao,* Rhonda Fleming, Bilaal McCloud, and Mark S. Klempner

Section of Infectious Diseases, Department of Medicine, Boston University Medical Center, Boston, Massachusetts 02118

Received 6 February 2007/ Returned for modification 13 March 2007/ Accepted 22 March 2007

Lyme disease is an infection caused by a tick-borne spirochete, Borrelia burgdorferi. Matrix metalloproteinase 9 (MMP-9) was selectively upregulated in the erythema migrans skin lesions of patients with acute Lyme disease. In this study, the mechanism of upregulation of MMP-9 was investigated in vitro and in vivo. The concentrations of MMP-9 and soluble CD14 were markedly elevated in serum from patients with acute Lyme disease and were also upregulated in U937 cells by B. burgdorferi in a time- and concentration-dependent manner. MMP-9 mRNA was expressed at baseline in fibroblasts in the presence or absence of B. burgdorferi. However, when fibroblasts were incubated with supernatants from U937 cells with B. burgdorferi or recombinant CD14, the expression of MMP-9 was significantly increased. This effect was completely abolished by the anti-CD14 antibody. These data suggest that the upregulation of MMP-9 by B. burgdorferi involves the CD14 pathway in infiltrating inflammatory cells. Fibroblasts could be recruited to amplify local production of MMP-9 by acquiring CD14 from macrophages.

* Corresponding author. Mailing address: Boston Medical Center, EBRC 6th Floor, 650 Albany St., Boston, MA 02118. Phone: (617) 414-7965. Fax: (617) 414-5280. E-mail: zhao{at}bu.edu

{triangledown} Published ahead of print on 2 April 2007.

Editor: R. P. Morrison

Infection and Immunity, June 2007, p. 3062-3069, Vol. 75, No. 6
0019-9567/07/$08.00+0     doi:10.1128/IAI.00202-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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