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Infection and Immunity, August 2007, p. 3715-3721, Vol. 75, No. 8
0019-9567/07/$08.00+0     doi:10.1128/IAI.00586-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Pseudomonas aeruginosa Forms Biofilms in Acute Infection Independent of Cell-to-Cell Signaling{triangledown} ,{dagger}

J. Andy Schaber,1,2 W. Jeffrey Triffo,3,4 Sang Jin Suh,5 Jeffrey W. Oliver,6 Mary Catherine Hastert,7 John A. Griswold,1 Manfred Auer,3 Abdul N. Hamood,2 and Kendra P. Rumbaugh1,7*

Departments of Surgery,1 Microbiology and Immunology,2 Pathology,6 Cell Biology and Biochemistry, Texas Tech University Health Sciences Center, 3601 4th St., Lubbock, Texas 79430,7 Life Sciences Division, Lawrence Berkeley National Laboratory, 1 Cyclotron Road, Berkeley, California 94720,3 Department of Bioengineering, Rice University, 6100 Main, Houston, Texas 77005,4 Department of Biological Sciences, Auburn University, 319 Life Sciences Building, Auburn, Alabama 368495

Received 23 April 2007/ Accepted 29 May 2007

Biofilms are bacterial communities residing within a polysaccharide matrix that are associated with persistence and antibiotic resistance in chronic infections. We show that the opportunistic pathogen Pseudomonas aeruginosa forms biofilms within 8 h of infection in thermally injured mice, demonstrating that biofilms contribute to bacterial colonization in acute infections as well. Using light, electron, and confocal scanning laser microscopy, P. aeruginosa biofilms were visualized within burned tissue surrounding blood vessels and adipose cells. Although quorum sensing (QS), a bacterial signaling mechanism, coordinates differentiation of biofilms in vitro, wild-type and QS-deficient P. aeruginosa strains formed similar biofilms in vivo. Our findings demonstrate that P. aeruginosa forms biofilms on specific host tissues independently of QS.

* Corresponding author. Mailing address: Texas Tech University Health Sciences Center, Department of Surgery, 3601 4th Street, Lubbock, TX 79430. Phone: (806) 743-2460, ext. 264. Fax: (806) 743-2370. E-mail: kendra.rumbaugh{at}ttuhsc.edu

{triangledown} Published ahead of print on 11 June 2007.

{dagger} Supplemental material for this article may be found at http://iai.asm.org/.

Editor: V. J. DiRita

Infection and Immunity, August 2007, p. 3715-3721, Vol. 75, No. 8
0019-9567/07/$08.00+0     doi:10.1128/IAI.00586-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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