This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kidd, S. P.
Right arrow Articles by McEwan, A. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kidd, S. P.
Right arrow Articles by McEwan, A. G.

 Previous Article  |  Next Article 

Infection and Immunity, September 2007, p. 4506-4513, Vol. 75, No. 9
0019-9567/07/$08.00+0     doi:10.1128/IAI.00487-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Glutathione-Dependent Alcohol Dehydrogenase AdhC Is Required for Defense against Nitrosative Stress in Haemophilus influenzae{triangledown}

Stephen P. Kidd, Donald Jiang, Michael P. Jennings, and Alastair G. McEwan*

Australian Bacterial Pathogenesis Program and Centre for Metals in Biology, School of Molecular and Microbial Sciences, University of Queensland, Brisbane, Queensland 4072, Australia

Received 5 April 2007/ Returned for modification 29 May 2007/ Accepted 15 June 2007

In Haemophilus influenzae Rd KW20, we identified a gene, adhC, which encodes a class III alcohol dehydrogenase (AdhC) and has S-nitrosoglutathione reductase activity. adhC exists on an operon with estD, which encodes an esterase. Divergent to the adhC-estD operon is the Haemophilus influenzae nmlR gene (nmlRHI), which encodes a MerR family regulator that is homologous to the Neisseria MerR-like regulator (NmlR). Analysis of an nmlRHI mutant indicated that expression of the adhC-estD operon is regulated by NmlRHI in strain Rd KW20. Chromosomal inactivation of either adhC or nmlRHI resulted in sensitivity to S-nitrosoglutathione and decreased S-nitrosoglutathione reductase activity. Examination of the NmlRHI-AdhC system in the genome sequences of nontypeable H. influenzae strains R2846, R2866, and 86-028NP identified significant variations. The adhC gene of 86-028NP was predicted to be nonfunctional due to a premature stop codon. Polymorphisms in the operator/promoter region of R2866 resulted in reduced enzyme activity. This correlated with an increased sensitivity to S-nitrosoglutathione. The adhC-nmlRHI system was examined in thirty-three clinical isolates (both capsular and nontypeable strains). Nucleic acid sequence data showed that only strain 86-028NP contained a premature stop codon. There were some variations in the DNA sequence of the operator/promoter region which altered the nmlRHI promoter. However, the clinical isolates still possessed S-nitrosoglutathione reductase activity and showed at least the equivalent ability to grow in the presence of S-nitrosoglutathione as Rd KW20. These data suggest that the nmlRHI-adhC system has a role in the defense against nitrosative stress in Haemophilus influenzae.

* Corresponding author. Mailing address: Australian Bacterial Pathogenesis Program and Centre for Metals in Biology, School of Molecular and Microbial Sciences, University of Queensland, Brisbane, Queensland 4072, Australia. Phone: 61 7 3365 4622. Fax: 61 7 3365 4273. E-mail: mcewan{at}uq.edu.au

{triangledown} Published ahead of print on 25 June 2007.

Editor: F. C. Fang

Infection and Immunity, September 2007, p. 4506-4513, Vol. 75, No. 9
0019-9567/07/$08.00+0     doi:10.1128/IAI.00487-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Harrington, J. C., Wong, S. M. S., Rosadini, C. V., Garifulin, O., Boyartchuk, V., Akerley, B. J. (2009). Resistance of Haemophilus influenzae to Reactive Nitrogen Donors and Gamma Interferon-Stimulated Macrophages Requires the Formate-Dependent Nitrite Reductase Regulator-Activated ytfE Gene. Infect. Immun. 77: 1945-1958 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»