This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sun, G.
Right arrow Articles by Yang, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sun, G.
Right arrow Articles by Yang, J.

 Previous Article  |  Next Article 

Infection and Immunity, October 2008, p. 4405-4413, Vol. 76, No. 10
0019-9567/08/$08.00+0     doi:10.1128/IAI.00575-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Mycoplasma pneumoniae Infection Induces Reactive Oxygen Species and DNA Damage in A549 Human Lung Carcinoma Cells{triangledown}

Gongping Sun,1 Xuefeng Xu,2 Yingshuo Wang,2 Xiaoyun Shen,1 Zhimin Chen,2* and Jun Yang1,3,4*

Department of Toxicology, Zhejiang University School of Public Health, Hangzhou, Zhejiang, 310058, China,1 The Children's Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, 310008, China,2 National Key Laboratory for Infectious Disease Diagnosis and Therapy, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, 310008, China,3 Zhejiang California International NanoSystems Institute, Hangzhou, Zhejiang, 310030, China4

Received 11 May 2008/ Returned for modification 13 June 2008/ Accepted 16 July 2008

Mycoplasma pneumoniae is a frequent cause of community-acquired bacterial respiratory infections in children and adults. In the present study, using a proteomic approach, we studied the effects of M. pneumoniae infection on the protein expression profile of A549 human lung carcinoma cells. M. pneumoniae infection induced changes in the expression of cellular proteins, in particular a group of proteins involved in the oxidative stress response, such as glucose-6-phosphate 1-dehydrogenase, NADH dehydrogenase (ubiquinone) Fe-S protein 2, and ubiquinol-cytochrome c reductase complex core protein I mitochondrial precursor. The oxidative status of M. pneumoniae-infected cells was evaluated, and the results revealed that M. pneumoniae infection indeed caused generation of reactive oxygen species (ROS). It was further shown that M. pneumoniae infection also induced DNA double-strand breaks, as demonstrated by the formation of {gamma}H2AX foci. On the other hand, an ROS scavenger, N-acetylcysteine, could inhibit the ROS generation, as well as decrease {gamma}H2AX focus formation. This is the first report showing that M. pneumoniae infection can directly induce DNA damage, at least partially, through the generation of ROS, and thus this report strengthens the powerful application of proteomics in the study of the pathogenesis of M. pneumoniae.

* Corresponding author. Mailing address for Jun Yang: National Key Laboratory for Infectious Disease Diagnosis and Therapy, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, 310008, China. Phone and fax: 86 571 88208140. E-mail: gastate{at}zju.edu.cn. Mailing address for Zhimin Chen: Department of Pediatric Pulmonology, The Children's Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, 310008, China. Phone: 86 571 87061007, ext. 70411. Fax: 86 571 87033296. E-mail: zmchen{at}zju.edu.cn

{triangledown} Published ahead of print on 28 July 2008.

Editor: F. C. Fang

Infection and Immunity, October 2008, p. 4405-4413, Vol. 76, No. 10
0019-9567/08/$08.00+0     doi:10.1128/IAI.00575-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»