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Infection and Immunity, October 2008, p. 4686-4691, Vol. 76, No. 10
0019-9567/08/$08.00+0     doi:10.1128/IAI.00238-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Binding of the Streptococcus gordonii DL1 Surface Protein Hsa to the Host Cell Membrane Glycoproteins CD11b, CD43, and CD50{triangledown}

Yumiko Urano-Tashiro,* Ayako Yajima, Eizo Takashima, Yukihiro Takahashi, and Kiyoshi Konishi

Department of Microbiology, School of Life Dentistry at Tokyo, Nippon Dental University, 1-9-20 Fujimi, Chiyoda-ku, Tokyo 102-8159, Japan

Received 20 February 2008/ Returned for modification 6 April 2008/ Accepted 24 July 2008

Infective endocarditis is frequently attributed to oral streptococci. The mechanisms of pathogenesis, however, are not well understood, although interaction between streptococci and phagocytes are thought to be very important. A highly expressed surface component of Streptococcus gordonii, Hsa, which has sialic acid-binding activity, contributes to infective endocarditis in vivo. In the present study, we found that S. gordonii DL1 binds to HL-60 cells differentiated into monocytes, granulocytes, and macrophages. Using a glutathione S-transferase (GST) fusion to the NR2 domain, which is the sialic acid-binding region of Hsa, we confirmed that the Hsa NR2 domain also binds to differentiated HL-60 cells. To identify which sialoglycoproteins on the surface of differentiated HL-60 cells are receptors for Hsa, intrinsic membrane proteins were assessed by bacterial overlay and far-Western blotting. S. gordonii DL1 adhered to 100- to 150-kDa proteins, a reaction that was abolished by neuraminidase treatment. These sialoglycoproteins were identified as CD11b, CD43, and CD50 by GST pull-down assay and immunoprecipitation with each specific monoclonal antibody. These data suggest that S. gordonii DL1 Hsa specifically binds to three glycoproteins as receptors and that this interaction may be the initial bacterial binding step in infective endocarditis by oral streptococci.

* Corresponding author. Mailing address: Department of Microbiology, School of Life Dentistry at Tokyo, Nippon Dental University, 1-9-20 Fujimi, Chiyoda-ku, Tokyo 102-8159, Japan. Phone and fax: 81-3-3261-8763. E-mail: yumiurano{at}tky.ndu.ac.jp

{triangledown} Published ahead of print on 4 August 2008.

Editor: A. Camilli

Infection and Immunity, October 2008, p. 4686-4691, Vol. 76, No. 10
0019-9567/08/$08.00+0     doi:10.1128/IAI.00238-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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