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Infection and Immunity, December 2008, p. 5581-5587, Vol. 76, No. 12
0019-9567/08/$08.00+0     doi:10.1128/IAI.00574-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Protection of Wild-Type and Severe Combined Immunodeficiency Mice against an Intranasal Challenge by Passive Immunization with Monoclonal Antibodies to the Chlamydia trachomatis Mouse Pneumonitis Major Outer Membrane Protein{triangledown}

Sukumar Pal, Jose Bravo, Ellena M. Peterson, and Luis M. de la Maza*

Department of Pathology and Laboratory Medicine, Medical Sciences, Room D440, University of California, Irvine, Irvine, California 92697-4800

Received 10 May 2008/ Returned for modification 3 July 2008/ Accepted 12 September 2008

Monoclonal antibodies (MAbs) to the Chlamydia trachomatis mouse pneumonitis (MoPn) major outer membrane protein (MOMP) were characterized for their ability to neutralize the infectivity of this organism in vitro and in vivo. One of the MAbs (MoPn-23) recognizes a nonlinear epitope in the MOMP, MAb MoPn-40 binds to a linear epitope in the variable domain 1 (VD1), and MAb MoPn-32 recognizes the chlamydial lipopolysaccharide. MAb MoPn-23 neutralized 50% of the infectivity of Chlamydia, as measured in vitro by using HAK (Fc{gamma}III–) and HeLa-229 (Fc{gamma}III+) cells at a concentration 100 times lower than MAb MoPn-40. MAb MoPn-32 had no neutralizing ability. In comparison to the control normal mouse immunoglobulin G, passive immunization of BALB/c mice with MAb MoPn-23 resulted in a highly significant protection against an intranasal (i.n.) challenge as determined by the change in body weight, the weight of the lungs, and the yield of Chlamydia inclusion-forming units (IFU) from the lungs. Passive immunization with MAb MoPn-40 resulted in a lower degree of protection, and MAb MoPn-32 afforded no protection. MAb MoPn-23 was also tested for its ability to protect wild-type (WT) and severe combined immunodeficient (SCID) C.B-17 mice against an i.n. challenge. Protection based on total body weight, lung weight, and yield of Chlamydia IFU was as effective in SCID as in WT C.B-17 mice. In conclusion, antibodies to MOMP can protect mice against a chlamydial infection in the presence or absence of T and B cells.

* Corresponding author. Mailing address: Department of Pathology and Laboratory Medicine, Medical Sciences, Rm. D440, University of California, Irvine, Irvine, CA 92697-4800. Phone: (949) 824-7450. Fax: (949) 824-2160. E-mail: lmdelama{at}uci.edu

{triangledown} Published ahead of print on 22 September 2008.

Editor: J. L. Flynn

Infection and Immunity, December 2008, p. 5581-5587, Vol. 76, No. 12
0019-9567/08/$08.00+0     doi:10.1128/IAI.00574-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



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