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Infection and Immunity, December 2008, p. 5655-5667, Vol. 76, No. 12
0019-9567/08/$08.00+0     doi:10.1128/IAI.00780-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Characterization of Two Campylobacter jejuni Strains for Use in Volunteer Experimental-Infection Studies ^,

Frédéric Poly,¹ Timothy D. Read,¹ Yu-Han Chen,² Mario A. Monteiro,² Oralak Serichantalergs,³ Piyarat Pootong,³ Ladaporn Bodhidatta,³ Carl J. Mason,³ David Rockabrand,¹ Shahida Baqar,¹ Chad K. Porter,¹ David Tribble,⁴ Michael Darsley,⁵ and Patricia Guerry^1*

Naval Medical Research Center, Silver Spring, Maryland,¹ University of Guelph, Guelph, Ontario, Canada,² Armed Forces Research Institute for Medical Sciences, Bangkok, Thailand,³ Uniformed Services University for the Health Sciences, Bethesda, Maryland,⁴ ACE Biosciences, Odense, Denmark⁵

Received 20 June 2008/ Returned for modification 23 June 2008/ Accepted 12 September 2008

The development of vaccines against Campylobacter jejuni would be facilitated by the ability to perform phase II challenge studies. However, molecular mimicry of the lipooligosaccharide (LOS) of most C. jejuni strains with human gangliosides presents safety concerns about the development of Guillain-Barré syndrome. Clinical isolates of C. jejuni that appeared to lack genes for the synthesis of ganglioside mimics were identified by DNA probe analyses. Two clinical isolates from Southeast Asia (strains BH-01-0142 and CG8421) were determined to express the LOS type containing N-acetyl quinovosamine. No ganglioside structures were observed to be present in the LOSs of these strains, and pyrosequence analyses of the genomes of both strains confirmed the absence of genes involved in ganglioside mimicry. The capsule polysaccharide (CPS) of BH-01-0142 was determined to be composed of galactose (Gal), 6-deoxy-ido-heptose, and, in smaller amounts, D-glycero-D-ido-heptose, and the CPS of CG8421 was observed to contain Gal, 6-deoxy-altro-heptose, N-acetyl-glucosamine, and minor amounts of 6-deoxy-3-O-Me-altro-heptose. Both CPSs were shown to carry O-methyl-phosphoramidate. The two genomes contained strain-specific zones, some of which could be traced to a plasmid origin, and both contained a large chromosomal insertion related to the CJEI3 element of C. jejuni RM1221. The genomes of both strains shared a high degree of similarity to each other and, with the exception of the capsule locus of CG8421, to the type strain of the HS3 serotype, TGH9011.

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* Corresponding author. Mailing address: Enteric Diseases Department, Naval Medical Research Center, 503 Robert Grant Avenue, Silver Spring, MD 20910. Phone: (301) 319-7662. Fax: (301) 319-7679. E-mail: patricia.guerry{at}med.navy.mil

Published ahead of print on 22 September 2008.

Supplemental material for this article may be found at http://iai.asm.org/.

Editor: S. R. Blanke

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Infection and Immunity, December 2008, p. 5655-5667, Vol. 76, No. 12
0019-9567/08/$08.00+0     doi:10.1128/IAI.00780-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

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