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Infection and Immunity, December 2008, p. 5714-5720, Vol. 76, No. 12
0019-9567/08/$08.00+0     doi:10.1128/IAI.00997-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Human {alpha}- and β-Defensins Bind to Immobilized Adhesins from Porphyromonas gingivalis{triangledown}

Deborah E. Dietrich,1 Xiangjun Xiao,1 Deborah V. Dawson,1 Myriam Bélanger,2 Hua Xie,3 Ann Progulske-Fox,2 and Kim A. Brogden1,4*

Dows Institute for Dental Research, College of Dentistry, The University of Iowa, Iowa City, Iowa 52242,1 Center for Molecular Biology and Department of Oral Biology, College of Dentistry, University of Florida, Gainesville, Florida 32610,2 School of Dentistry, Meharry Medical College, Nashville, Tennessee,3 Department of Periodontics, College of Dentistry, The University of Iowa, Iowa City, Iowa 522424

Received 8 August 2008/ Returned for modification 9 September 2008/ Accepted 3 October 2008

Human neutrophil peptide {alpha}-defensins (HNPs) and human β-defensins (HBDs) are small well-characterized peptides with broad antimicrobial activities and a diversity of innate immune functions. Although the interactions of defensins with bacteria and their membranes have been well characterized, the interactions of defensins with bacterial adhesins have not. Here we determine if HNPs and HBDs bind to the immobilized adhesins of Porphyromonas gingivalis strain 381, recombinant hemagglutinin B (rHagB) and recombinant fimbrillin A (rFimA), by surface plasmon resonance spectroscopy. Association of HNPs and HBDs with rHagB or rFimA was dose dependent and defensin specific. HBD3, HNP-2, and HNP-1 bound more readily to immobilized rHagB than HBD2 and HBD1 did. HNP-2, HNP-1, and HBD3 bound more readily to immobilized rFimA than HBD1 and HBD2 did. Binding of defensins to adhesins may serve to prevent microbial adherence to tissues, attenuate proinflammatory cytokine responses, and facilitate delivery of bound antigen to antigen-presenting cells with defensin receptors.

* Corresponding author. Mailing address: Department of Periodontics and Dows Institute for Dental Research, N447 DSB, College of Dentistry, 801 Newton Road, The University of Iowa, Iowa City, IA 52242. Phone: (319) 335-8077. Fax: (319) 335-8895. E-mail: kim-brogden{at}uiowa.edu

{triangledown} Published ahead of print on 13 October 2008.

Editor: F. C. Fang

Infection and Immunity, December 2008, p. 5714-5720, Vol. 76, No. 12
0019-9567/08/$08.00+0     doi:10.1128/IAI.00997-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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