This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental material
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Forman, S.
Right arrow Articles by Straley, S. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Forman, S.
Right arrow Articles by Straley, S. C.

 Previous Article  |  Next Article 

Infection and Immunity, February 2008, p. 578-587, Vol. 76, No. 2
0019-9567/08/$08.00+0     doi:10.1128/IAI.00219-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

yadBC of Yersinia pestis, a New Virulence Determinant for Bubonic Plague{triangledown} ,{dagger}

Stanislav Forman, Christine R. Wulff,{ddagger} Tanya Myers-Morales, Clarissa Cowan, Robert D. Perry, and Susan C. Straley*

Department of Microbiology, Immunology, and Molecular Genetics, University of Kentucky, Lexington, Kentucky 40536-0298

Received 9 February 2007/ Returned for modification 16 March 2007/ Accepted 23 October 2007

In all Yersinia pestis strains examined, the adhesin/invasin yadA gene is a pseudogene, yet Y. pestis is invasive for epithelial cells. To identify potential surface proteins that are structurally and functionally similar to YadA, we searched the Y. pestis genome for open reading frames with homology to yadA and found three: the bicistronic operon yadBC (YPO1387 and YPO1388 of Y. pestis CO92; y2786 and y2785 of Y. pestis KIM5), which encodes two putative surface proteins, and YPO0902, which lacks a signal sequence and likely is nonfunctional. In this study we characterized yadBC regulation and tested the importance of this operon for Y. pestis adherence, invasion, and virulence. We found that loss of yadBC caused a modest loss of invasiveness for epithelioid cells and a large decrease in virulence for bubonic plague but not for pneumonic plague in mice.

* Corresponding author. Mailing address: Department of Microbiology, Immunology, and Molecular Genetics, MS415 Medical Center, University of Kentucky, Lexington, KY 40536-0298. Phone: (859) 323-6538. Fax: (859) 257-8994. E-mail: scstra01{at}uky.edu

{triangledown} Published ahead of print on 19 November 2007.

{dagger} Supplemental material for this article may be found at http://iai.asm.org/.

Editor: D. L. Burns

{ddagger} Present address: Lutheran Theological Southern Seminary, Columbia, SC.

Infection and Immunity, February 2008, p. 578-587, Vol. 76, No. 2
0019-9567/08/$08.00+0     doi:10.1128/IAI.00219-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Ye, Z., Kerschen, E. J., Cohen, D. A., Kaplan, A. M., van Rooijen, N., Straley, S. C. (2009). Gr1+ Cells Control Growth of YopM-Negative Yersinia pestis during Systemic Plague. Infect. Immun. 77: 3791-3806 [Abstract] [Full Text]  
  • Zhou, D., Yang, R. (2009). Molecular Darwinian Evolution of Virulence in Yersinia pestis. Infect. Immun. 77: 2242-2250 [Full Text]  
  • Anisimov, A. P., Bakhteeva, I. V., Panfertsev, E. A., Svetoch, T. E., Kravchenko, T. B., Platonov, M. E., Titareva, G. M., Kombarova, T. I., Ivanov, S. A., Rakin, A. V., Amoako, K. K., Dentovskaya, S. V. (2009). The subcutaneous inoculation of pH 6 antigen mutants of Yersinia pestis does not affect virulence and immune response in mice. J Med Microbiol 58: 26-36 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»