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Infection and Immunity, February 2008, p. 658-663, Vol. 76, No. 2
0019-9567/08/$08.00+0     doi:10.1128/IAI.01291-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Transcriptome Changes in Mycoplasma hyopneumoniae during Infection{triangledown} ,{dagger}

Melissa L. Madsen, Supraja Puttamreddy, Eileen L. Thacker, Michael D. Carruthers, and F. Chris Minion*

Department of Veterinary Microbiology and Preventive Medicine, Iowa State University, Ames, Iowa

Received 21 September 2007/ Returned for modification 31 October 2007/ Accepted 22 November 2007

Mycoplasma hyopneumoniae causes swine pneumonia and contributes significantly to the porcine respiratory disease complex. The mechanisms of pathogenesis are difficult to address, since there is a lack of genetic tools, but microarrays are available and can be used to study transcriptional changes that occur during disease as a way to identify important virulence-related genes. Mycoplasmas were collected from bronchial alveolar lavage samples and compared to broth-grown cells using microarrays. Bronchial alveolar lavage was performed on pigs 28 days postinfection, and mycoplasmas were isolated by differential centrifugation. Mycoplasma RNA-enriched preparations were then obtained from total RNA by subtracting eucaryotic ribosomal and messenger RNAs. Labeled cDNAs were generated with mycoplasma open reading frame-specific primers. Nine biological replicates were analyzed. During lung infection, our analysis indicated that 79 M. hyopneumoniae genes were differentially expressed (P < 0.01), at a false-discovery rate of <2.7%. Of the down-regulated genes, 28 of 46 (61%) lacked an assigned function, in comparison to 21 of 33 (63%) of up-regulated genes. Four down-regulated genes and two up-regulated genes encoded putative lipoproteins. secA (mhp295) (P = 0.003) and two glycerol transport permease genes (potA [mhp380; P = 0.006] and ugpA [mhp381; P = 0.003]) were up-regulated in vivo. Elongation factor EF-G (fusA [mhp083]) (P = 0.002), RNA polymerase beta chain (rpoC [mhp635]) (P = 0.003), adenylate kinase (adk [mhp208]) (P = 0.001), prolyl aminoacyl tRNA synthetase (proS [mhp397]) (P = 0.009), and cysteinyl-tRNA synthetase (cysS [mhp661]) (P < 0.001) were down-regulated in vivo.

* Corresponding author. Mailing address: Department of Veterinary Microbiology and Preventive Medicine, Iowa State University, Ames, IA 50011. Phone: (515) 294-6347. Fax: (515) 294-1401. E-mail: fcminion{at}iastate.edu

{triangledown} Published ahead of print on 10 December 2007.

{dagger} Supplemental material for this article may be found at http://iai.asm.org/.

Editor: A. Camilli

Infection and Immunity, February 2008, p. 658-663, Vol. 76, No. 2
0019-9567/08/$08.00+0     doi:10.1128/IAI.01291-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Oneal, M. J., Schafer, E. R., Madsen, M. L., Minion, F. C. (2008). Global transcriptional analysis of Mycoplasma hyopneumoniae following exposure to norepinephrine. Microbiology 154: 2581-2588 [Abstract] [Full Text]  


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