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Infection and Immunity, February 2008, p. 717-725, Vol. 76, No. 2
0019-9567/08/$08.00+0     doi:10.1128/IAI.00974-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Global Transcriptional Profile of Mycobacterium tuberculosis during THP-1 Human Macrophage Infection{triangledown} ,{dagger}

Patricia Fontán,1 Virginie Aris,2 Saleena Ghanny,2 Patricia Soteropoulos,2 and Issar Smith1*

TB Center,1 Center for Applied Genomics, The Public Health Research Institute, UMDNJ-New Jersey Medical School, 225 Warren Street, Newark, New Jersey 07103-35352

Received 17 July 2007/ Returned for modification 25 September 2007/ Accepted 27 November 2007

During lung infection, Mycobacterium tuberculosis resides in macrophages and subverts the bactericidal mechanisms of these professional phagocytes. Comprehension of this host-pathogen relationship is fundamental for the development of new therapies to cure and prevent tuberculosis. In this work, we analyzed the transcriptional profile of M. tuberculosis infecting human macrophage-like THP-1 cells in order to identify putative bacterial pathogenic factors that can be relevant for the intracellular survival of M. tuberculosis. We compared the gene expression profile of M. tuberculosis H37Rv after 4 h and 24 h of infection of human macrophage-like THP-1 cells with the gene expression profile of the strain growing exponentially in broth cultures. We found 585 genes expressed differentially by intracellular M. tuberculosis. An analysis of the gene expression profile of M. tuberculosis inside THP-1 cells suggests the perturbation of the cell envelope as a major intracellular stress inside THP-1 macrophages.

* Corresponding author. Mailing address: Room W210W, 225 Warren Street, Newark, NJ 07103-3535. Phone: (973) 854-3260. Fax: (973) 854-3101. E-mail: smithis{at}umdnj.edu

{triangledown} Published ahead of print on 10 December 2007.

{dagger} Supplemental material for this article may be found at http://iai.asm.org/.

Editor: F. C. Fang

Infection and Immunity, February 2008, p. 717-725, Vol. 76, No. 2
0019-9567/08/$08.00+0     doi:10.1128/IAI.00974-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.





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