This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Cai, X.
Right arrow Articles by Lu, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Cai, X.
Right arrow Articles by Lu, C.

 Previous Article  |  Next Article 

Infection and Immunity, February 2008, p. 767-770, Vol. 76, No. 2
0019-9567/08/$08.00+0     doi:10.1128/IAI.00444-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Effective Production and Purification of the Glycosylated TSOL18 Antigen, Which Is Protective against Pig Cysticercosis{triangledown}

Xuepeng Cai,1,{dagger}* Gailing Yuan,1,{dagger}* Yadong Zheng,1,{dagger} Xuenong Luo,1 Shaohua Zhang,1 Juntao Ding,1 Zhizhong Jing,1 and Chengping Lu2

Key Laboratory of Zoonoses of CAAS, Key Laboratory of Veterinary Parasitology of Gansu Province, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, CAAS, Lanzhou 730046, Gansu,1 Nanjing Agricultural University, Weigang 210095, Nanjing, China2

Received 27 March 2007/ Returned for modification 9 May 2007/ Accepted 16 November 2007

Cysticercosis caused by Taenia solium metacestodes is a worldwide public health problem. Important progress in the development of effective and practical vaccines against this disease has been made. In this study, the promising T. solium oncospheral vaccine candidate named TSOL18 antigen was produced in a 5-liter fermentor. During the process of fermentation, the pH of the culture was always kept below 5.0, and in order to prevent foaming, an antifoam agent was added. In addition, the oxygen content of the culture was constantly kept at >50% in our experiment. A high level of the glycosylated protein (2.5 g/liter) was obtained, and the protein was easily purified by gel chromatography. Vaccination trials showed that the recombinant TSOL18 antigen induced 94 and 100% reductions in metacestode burdens in vaccinated pigs, obviously higher than the 89% reduction in pigs immunized with cysticercus crude extracts in trial 1. These are very promising results in the development of an efficient tool to control cysticercosis in Asia.

* Corresponding author. Mailing address: Xujiaping 1, Yanchangbu, Lanzhou 730046, Gansu, People's Republic of China. Phone: 86-931-8342716. Fax: 86-931-8340977. E-mail for Xuepeng Cai: caixp{at}public.lz.gs.cn. E-mail for Gailing Yuan: zhyd9{at}hotmail.com

{triangledown} Published ahead of print on 10 December 2007.

Editor: W. A. Petri, Jr.

{dagger} These authors equally contributed to this work.

Infection and Immunity, February 2008, p. 767-770, Vol. 76, No. 2
0019-9567/08/$08.00+0     doi:10.1128/IAI.00444-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Robinson, M. W., Dalton, J. P. (2009). Zoonotic helminth infections with particular emphasis on fasciolosis and other trematodiases. Phil Trans R Soc B 364: 2763-2776 [Abstract] [Full Text]  
  • Luo, X., Zheng, Y., Hou, J., Zhang, S., Cai, X. (2009). Protection against Asiatic Taenia solium Induced by a Recombinant 45W-4B Protein. CVI 16: 230-232 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»