This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lindberg, S.
Right arrow Articles by Uhlin, B. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lindberg, S.
Right arrow Articles by Uhlin, B. E.

 Previous Article  |  Next Article 

Infection and Immunity, February 2008, p. 771-780, Vol. 76, No. 2
0019-9567/08/$08.00+0     doi:10.1128/IAI.01010-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Regulatory Interactions among Adhesin Gene Systems of Uropathogenic Escherichia coli{triangledown}

Stina Lindberg,1,{dagger} Yan Xia,1,{dagger},{ddagger} Berit Sondén,1 Mikael Göransson,1 Jörg Hacker,2 and Bernt Eric Uhlin1*

Department of Molecular Biology and Laboratory for Molecular Infection Medicine Sweden (MIMS), Umeå University, S-90187 Umeå, Sweden,1 Institut für Molekulare Infektionsbiologie, Röntgenring 11, 97070 Würzburg, Germany2

Received 24 July 2007/ Returned for modification 2 September 2007/ Accepted 13 November 2007

Uropathogenic Escherichia coli strain J96 carries multiple determinants for fimbrial adhesins. The regulatory protein PapB of P fimbriae has previously been implicated in potential coregulatory events. The focB gene of the F1C fimbria determinant is highly homologous to papB; the translated sequences share 81% identity. In this study we investigated the role of PapB and FocB in regulation of the F1C fimbriae. By using gel mobility shift assays, we showed that FocB binds to sequences in both the pap and foc operons in a somewhat different manner than PapB. The results of both in vitro cross-linking and in vivo oligomerization tests indicated that FocB could function in an oligomeric fashion. Furthermore, our results suggest that PapB and FocB can form heterodimers and that these complexes can repress expression of the foc operon. The effect of FocB on expression of type 1 fimbriae was also tested. Taken together, the results that we present expand our knowledge about a regulatory network for different adhesin gene systems in uropathogenic E. coli and suggest a hierarchy for expression of the fimbrial adhesins.

* Corresponding author. Mailing address: Department of Molecular Biology and Laboratory for Molecular Infection Medicine Sweden (MIMS), Umeå University, S-90187 Umeå, Sweden. Phone: 46-90-7856731. Fax: 46-90-772630. E-mail: bernt.eric.uhlin{at}molbiol.umu.se

{triangledown} Published ahead of print on 26 November 2007.

Editor: A. J. Bäumler

{dagger} S.L. and Y.X. contributed equally to this work.

{ddagger} Present address: Department of Neuro-Oncology, MD Anderson Cancer Center, Houston, TX 77030.

Infection and Immunity, February 2008, p. 771-780, Vol. 76, No. 2
0019-9567/08/$08.00+0     doi:10.1128/IAI.01010-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Struve, C., Bojer, M., Krogfelt, K. A. (2009). Identification of a Conserved Chromosomal Region Encoding Klebsiella pneumoniae Type 1 and Type 3 Fimbriae and Assessment of the Role of Fimbriae in Pathogenicity. Infect. Immun. 77: 5016-5024 [Abstract] [Full Text]  
  • Simms, A. N., Mobley, H. L. T. (2008). PapX, a P Fimbrial Operon-Encoded Inhibitor of Motility in Uropathogenic Escherichia coli. Infect. Immun. 76: 4833-4841 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»