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Infection and Immunity, April 2008, p. 1476-1484, Vol. 76, No. 4
0019-9567/08/$08.00+0     doi:10.1128/IAI.01286-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Attenuated Endocytosis and Toxicity of a Mutant Cholera Toxin with Decreased Ability To Cluster Ganglioside GM₁ Molecules

Anne A. Wolf,¹ Michael G. Jobling,² David E. Saslowsky,¹ Eli Kern,¹ Kimberly R. Drake,⁴ Anne K. Kenworthy,⁴ Randall K. Holmes,² and Wayne I. Lencer^1,3*

GI Cell Biology, Department of Pediatrics, Children's Hospital and Harvard Medical School,¹ Harvard Digestive Diseases Center, Boston, Massachusetts 02115,³ Department of Microbiology, University of Colorado Denver School of Medicine, Aurora, Colorado 80045,² Departments of Molecular Physiology and Biophysics and Cell and Developmental Biology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232⁴

Received 20 September 2007/ Returned for modification 6 November 2007/ Accepted 9 January 2008

Cholera toxin (CT) moves from the plasma membrane (PM) of host cells to the endoplasmic reticulum (ER) by binding to the lipid raft ganglioside GM₁. The homopentomeric B-subunit of the toxin can bind up to five GM₁ molecules at once. Here, we examined the role of polyvalent binding of GM₁ in CT action by producing chimeric CTs that had B-subunits with only one or two normal binding pockets for GM₁. The chimeric toxins had attenuated affinity for binding to host cell PM, as expected. Nevertheless, like wild-type (wt) CT, the CT chimeras induced toxicity, fractionated with detergent-resistant membranes extracted from toxin-treated cells, displayed restricted diffusion in the plane of the PM in intact cells, and remained bound to GM₁ when they were immunoprecipitated. Thus, binding normally to two or perhaps only one GM₁ molecule is sufficient for association with lipid rafts in the PM and toxin action. The chimeric toxins, however, were much less potent than wt toxin, and they entered the cell by endocytosis more slowly, suggesting that clustering of GM₁ molecules by the B-subunit enhances the efficiency of toxin uptake and perhaps also trafficking to the ER.

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* Corresponding author. Mailing address: GI Cell Biology, Enders 720, Children's Hospital Boston, 300 Longwood Ave., Boston, MA 02115. Phone: (617) 919-2573. Fax: (617) 730-0498. E-mail: wayne.lencer{at}childrens.harvard.edu

Published ahead of print on 22 January 2008.

Editor: S. R. Blanke

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Infection and Immunity, April 2008, p. 1476-1484, Vol. 76, No. 4
0019-9567/08/$08.00+0     doi:10.1128/IAI.01286-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

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