IAI Track the topics, authors and articles important to you
Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Other Versions of this Article:
IAI.01424-07v1
76/5/1848    most recent
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Google Scholar
Right arrow Articles by Brinkman, M. B.
Right arrow Articles by Palzkill, T.
PubMed
Right arrow PubMed Citation
Right arrow Articles by Brinkman, M. B.
Right arrow Articles by Palzkill, T.
Infection and Immunity, May 2008, p. 1848-1857, Vol. 76, No. 5
0019-9567/08/$08.00+0     doi:10.1128/IAI.01424-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

A Novel Treponema pallidum Antigen, TP0136, Is an Outer Membrane Protein That Binds Human Fibronectin{triangledown}

Mary Beth Brinkman,1 Melanie A. McGill,2 Jonas Pettersson,3 Arthur Rogers,3 Petra Matejková,5 David Smajs,5 George M. Weinstock,6 Steven J. Norris,2,4 and Timothy Palzkill1,3*

Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, One Baylor Plaza, Houston, Texas 77030,1 Department of Pathology and Laboratory Medicine, University of Texas—Houston Medical School, 6431 Fannin Street, Houston, Texas 77030,2 Department of Molecular Virology & Microbiology, Baylor College of Medicine, One Baylor Plaza, Houston, Texas 77030,3 Department of Microbiology and Molecular Genetics, University of Texas—Houston Medical School, 6431 Fannin Street, Houston, Texas 77030,4 Department of Biology, Faculty of Medicine, Masaryk University, Kamenica 5, Building 6, 625 00 Brno, Czech Republic,5 Human Genome Sequencing Center, Baylor College of Medicine, One Baylor Plaza, Houston, Texas 770306

Received 23 October 2007/ Returned for modification 21 December 2007/ Accepted 25 February 2008

The antigenicity, structural location, and function of the predicted lipoprotein TP0136 of Treponema pallidum subsp. pallidum were investigated based on previous screening studies indicating that anti-TP0136 antibodies are present in the sera of syphilis patients and experimentally infected rabbits. Recombinant TP0136 (rTP0136) protein was purified and shown to be strongly antigenic during human and experimental rabbit infection. The TP0136 protein was exposed on the surface of the bacterial outer membrane and bound to the host extracellular matrix glycoproteins fibronectin and laminin. In addition, the TP0136 open reading frame was shown to be highly polymorphic among T. pallidum subspecies and strains at the nucleotide and amino acid levels. Finally, the ability of rTP0136 protein to act as a protective antigen to subsequent challenge with infectious T. pallidum in the rabbit model of infection was assessed. Immunization with rTP0136 delayed ulceration but did not prevent infection or the formation of lesions. These results demonstrate that TP0136 is expressed on the outer membrane of the treponeme during infection and may be involved in attachment to host extracellular matrix components.


* Corresponding author. Mailing address: Department of Molecular Virology & Microbiology, Baylor College of Medicine, One Baylor Place, Houston, TX 77030. Phone: (713) 798-5609. Fax: (713) 798-7375. E-mail: timothyp{at}bcm.tmc.edu

{triangledown} Published ahead of print on 10 March 2008.

Editor: R. P. Morrison


Infection and Immunity, May 2008, p. 1848-1857, Vol. 76, No. 5
0019-9567/08/$08.00+0     doi:10.1128/IAI.01424-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.







Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
J. Bacteriol. J. Virol. Eukaryot. Cell
Microbiol. Mol. Biol. Rev. Clin. Vaccine Immunol. All ASM Journals

Copyright © 2008 by the American Society for Microbiology. All rights reserved.