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Infection and Immunity, May 2008, p. 1970-1978, Vol. 76, No. 5
0019-9567/08/$08.00+0     doi:10.1128/IAI.00714-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

A Tightly Regulated Surface Protein of Borrelia burgdorferi Is Not Essential to the Mouse-Tick Infectious Cycle{triangledown}

Philip E. Stewart,* Aaron Bestor, Jonah N. Cullen, and Patricia A. Rosa

Laboratory of Zoonotic Pathogens, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 903 South 4th St., Hamilton, Montana 59840

Received 25 May 2007/ Returned for modification 21 June 2007/ Accepted 27 February 2008

Borrelia burgdorferi synthesizes a variety of differentially regulated outer surface lipoproteins in the tick vector and in vertebrate hosts. Among these is OspD, a protein that is highly induced in vitro by conditions that mimic the tick environment. Using genetically engineered strains in which ospD is deleted, we demonstrate that this protein is not required for B. burgdorferi survival and infectivity in either the mouse or the tick. However, examination of both transcript levels and protein expression indicates that OspD expression is limited to a discrete window of time during B. burgdorferi replication within the tick. This time frame corresponds to tick detachment from the host following feeding, and expression of OspD continues during tick digestion of the blood meal but is low or undetectable after the tick has molted. The high level of OspD production correlates to the highest cell densities that B. burgdorferi is known to reach in vivo. Although OspD is nonessential to the infectious cycle of B. burgdorferi, the tight regulation of expression suggests a beneficial contribution of OspD to the spirochete during bacterial replication within the tick midgut.

* Corresponding author. Mailing address: Rocky Mountain Laboratories, NIAID, NIH, 903 South 4th St., Hamilton, MT 59840. Phone: (406) 363-9301. Fax: (406) 363-9445. E-mail: pestewart{at}niaid.nih.gov

{triangledown} Published ahead of print on 10 March 2008.

Editor: D. L. Burns

Infection and Immunity, May 2008, p. 1970-1978, Vol. 76, No. 5
0019-9567/08/$08.00+0     doi:10.1128/IAI.00714-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Mulay, V. B., Caimano, M. J., Iyer, R., Dunham-Ems, S., Liveris, D., Petzke, M. M., Schwartz, I., Radolf, J. D. (2009). Borrelia burgdorferi bba74 Is Expressed Exclusively during Tick Feeding and Is Regulated by Both Arthropod- and Mammalian Host-Specific Signals. J. Bacteriol. 191: 2783-2794 [Abstract] [Full Text]  


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