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Infection and Immunity, May 2008, p. 2063-2069, Vol. 76, No. 5
0019-9567/08/$08.00+0     doi:10.1128/IAI.01643-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

LipL32 Is an Extracellular Matrix-Interacting Protein of Leptospira spp. and Pseudoalteromonas tunicata

David E. Hoke,¹ Suhelen Egan,² Paul A. Cullen,¹ and Ben Adler^1,3*

Australian Bacterial Pathogenesis Program,¹ Australian Research Council Centre of Excellence in Structural and Functional Microbial Genomics, Department of Microbiology, Monash University, VIC 3800, Australia,³ School of Biotechnology and Biomolecular Sciences, Centre for Marine Bio-Innovation, University of New South Wales, NSW 2052, Australia²

Received 11 December 2007/ Returned for modification 8 January 2008/ Accepted 10 February 2008

LipL32 is the major outer membrane protein in pathogenic Leptospira. It is highly conserved throughout pathogenic species and is expressed in vivo during human infection. While these data suggest a role in pathogenesis, a function for LipL32 has not been defined. Outer membrane proteins of gram-negative bacteria are the first line of molecular interaction with the host, and many have been shown to bind host extracellular matrix (ECM). A search for leptospiral ECM-interacting proteins identified the major outer membrane protein, LipL32. To verify this finding, recombinant LipL32 was expressed in Escherichia coli and was found to bind Matrigel ECM and individual components of ECM, including laminin, collagen I, and collagen V. Likewise, an orthologous protein found in the genome of Pseudoalteromonas tunicata strain D2 was expressed and found to be functionally similar and immunologically cross-reactive. Lastly, binding activity was mapped to the C-terminal 72 amino acids. These studies show that LipL32 and an orthologous protein in P. tunicata are immunologically cross-reactive and function as ECM-interacting proteins via a conserved C-terminal region.

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* Corresponding author. Mailing address: Monash University, VIC 3800, Australia. Phone: 61 3 9905 4815. Fax: 61 3 9905 4811. E-mail: Ben.Adler{at}med.monash.edu.au

Published ahead of print on 19 February 2008.

Editor: J. B. Bliska

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Infection and Immunity, May 2008, p. 2063-2069, Vol. 76, No. 5
0019-9567/08/$08.00+0     doi:10.1128/IAI.01643-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

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