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Infection and Immunity, May 2008, p. 2249-2255, Vol. 76, No. 5
0019-9567/08/$08.00+0     doi:10.1128/IAI.00024-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Vaccine-Elicited 10-Kilodalton Culture Filtrate Protein-Specific CD8+ T Cells Are Sufficient To Mediate Protection against Mycobacterium tuberculosis Infection{triangledown}

Ying Wu,1 Joshua S. Woodworth,1 Daniel S. Shin,1 Sheldon Morris,2 and Samuel M. Behar1*

Division of Rheumatology, Immunology, and Allergy, Brigham and Women's Hospital and Harvard Medical School, Boston, Massachusetts 02115,1 Laboratory of Mycobacterial Diseases and Cellular Immunology, FDA/CBER, Bethesda, Maryland 208922

Received 7 January 2008/ Returned for modification 23 February 2008/ Accepted 28 February 2008

The 10-kDa culture filtrate protein (CFP-10) and 6-kDa early secretory antigen of T cells (ESAT-6) are secreted in abundance by Mycobacterium tuberculosis and are frequently recognized by T cells from infected people. The genes encoding these proteins have been deleted from the genome of the vaccine strain Mycobacterium bovis bacillus Calmette-Guérin (BCG), and it is hypothesized that these proteins are important targets of protective immunity. Indeed, vaccination with ESAT-6 elicits protective CD4+ T cells in C57BL/6 mice. We have previously shown that M. tuberculosis infection of C3H mice elicits CFP-10-specific CD8+ and CD4+ T cells. Here we demonstrate that immunization with a CFP-10 DNA vaccine stimulates a specific T-cell response only to the H-2Kk-restricted epitope CFP-1032-39. These CFP-1032-39-specific CD8+ cells undergo a rapid expansion and accumulate in the lung following challenge of immunized mice with aerosolized M. tuberculosis. Protective immunity is induced by CFP-10 DNA vaccination as measured by a CFU reduction in the lung and spleen 4 and 8 weeks after challenge with M. tuberculosis. These data demonstrate that CFP-10 is a protective antigen and that CFP-1032-39-specific CD8+ T cells elicited by vaccination are sufficient to mediate protection against tuberculosis.


* Corresponding author. Mailing address: Division of Rheumatology, Immunology and Allergy, Brigham and Women's Hospital, Smith Building Room 516C, One Jimmy Fund Way, Boston, MA 02115. Phone: (617) 525-1033. Fax: (617) 525-1010. E-mail: sbehar{at}rics.bwh.harvard.edu

{triangledown} Published ahead of print on 10 March 2008.

Editor: J. L. Flynn


Infection and Immunity, May 2008, p. 2249-2255, Vol. 76, No. 5
0019-9567/08/$08.00+0     doi:10.1128/IAI.00024-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.







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