This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hasegawa, Y.
Right arrow Articles by Lamont, R. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hasegawa, Y.
Right arrow Articles by Lamont, R. J.

 Previous Article  |  Next Article 

Infection and Immunity, June 2008, p. 2420-2427, Vol. 76, No. 6
0019-9567/08/$08.00+0     doi:10.1128/IAI.00156-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Role of Porphyromonas gingivalis SerB in Gingival Epithelial Cell Cytoskeletal Remodeling and Cytokine Production{triangledown}

Yoshiaki Hasegawa,1,{dagger} Gena D. Tribble,1,{ddagger} Henry V. Baker,2 Jeffrey J. Mans,1 Martin Handfield,1 and Richard J. Lamont1*

Department of Oral Biology and Center for Molecular Microbiology, College of Dentistry,1 Department of Molecular Genetics and Microbiology, College of Medicine, University of Florida, Gainesville, Florida2

Received 5 February 2008/ Returned for modification 11 March 2008/ Accepted 18 March 2008

The SerB protein of Porphyromonas gingivalis is a HAD family serine phosphatase that plays a critical role in entry and survival of the organism in gingival epithelial cells. SerB is secreted by P. gingivalis upon contact with epithelial cells. Here it is shown by microarray analysis that SerB impacts the transcriptional profile of gingival epithelial cells, with pathways involving the actin cytoskeleton and cytokine production among those significantly overpopulated with differentially regulated genes. Consistent with the transcriptional profile, a SerB mutant of P. gingivalis exhibited defective remodeling of actin in epithelial cells. Interaction between gingival epithelial cells and isolated SerB protein resulted in actin rearrangement and an increase in the F/G actin ratio. SerB protein was also required for P. gingivalis to antagonize interleukin-8 accumulation following stimulation of epithelial cells with Fusobacterium nucleatum. SerB is thus capable of modulating host cell signal transduction that impacts the actin cytoskeleton and cytokine production.

* Corresponding author. Mailing address: Department of Oral Biology and Center for Molecular Microbiology, College of Dentistry, University of Florida, Gainesville, FL 32610-0424. Phone: (352) 392-5067. Fax: (352) 392-2361. E-mail: rlamont{at}dental.ufl.edu

{triangledown} Published ahead of print on 7 April 2008.

Editor: J. B. Bliska

{dagger} Present address: Department of Microbiology, School of Dentistry, Aichi-Gakuin University, Nagoya, Japan.

{ddagger} Present address: Department of Periodontics, University of Texas Dental Branch, Houston, TX.

Infection and Immunity, June 2008, p. 2420-2427, Vol. 76, No. 6
0019-9567/08/$08.00+0     doi:10.1128/IAI.00156-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Yilmaz, O. (2008). The chronicles of Porphyromonas gingivalis: the microbium, the human oral epithelium and their interplay. Microbiology 154: 2897-2903 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»