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Infection and Immunity, June 2008, p. 2420-2427, Vol. 76, No. 6
0019-9567/08/$08.00+0     doi:10.1128/IAI.00156-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Role of Porphyromonas gingivalis SerB in Gingival Epithelial Cell Cytoskeletal Remodeling and Cytokine Production{triangledown}

Yoshiaki Hasegawa,1,{dagger} Gena D. Tribble,1,{ddagger} Henry V. Baker,2 Jeffrey J. Mans,1 Martin Handfield,1 and Richard J. Lamont1*

Department of Oral Biology and Center for Molecular Microbiology, College of Dentistry,1 Department of Molecular Genetics and Microbiology, College of Medicine, University of Florida, Gainesville, Florida2

Received 5 February 2008/ Returned for modification 11 March 2008/ Accepted 18 March 2008

The SerB protein of Porphyromonas gingivalis is a HAD family serine phosphatase that plays a critical role in entry and survival of the organism in gingival epithelial cells. SerB is secreted by P. gingivalis upon contact with epithelial cells. Here it is shown by microarray analysis that SerB impacts the transcriptional profile of gingival epithelial cells, with pathways involving the actin cytoskeleton and cytokine production among those significantly overpopulated with differentially regulated genes. Consistent with the transcriptional profile, a SerB mutant of P. gingivalis exhibited defective remodeling of actin in epithelial cells. Interaction between gingival epithelial cells and isolated SerB protein resulted in actin rearrangement and an increase in the F/G actin ratio. SerB protein was also required for P. gingivalis to antagonize interleukin-8 accumulation following stimulation of epithelial cells with Fusobacterium nucleatum. SerB is thus capable of modulating host cell signal transduction that impacts the actin cytoskeleton and cytokine production.

* Corresponding author. Mailing address: Department of Oral Biology and Center for Molecular Microbiology, College of Dentistry, University of Florida, Gainesville, FL 32610-0424. Phone: (352) 392-5067. Fax: (352) 392-2361. E-mail: rlamont{at}dental.ufl.edu

{triangledown} Published ahead of print on 7 April 2008.

Editor: J. B. Bliska

{dagger} Present address: Department of Microbiology, School of Dentistry, Aichi-Gakuin University, Nagoya, Japan.

{ddagger} Present address: Department of Periodontics, University of Texas Dental Branch, Houston, TX.

Infection and Immunity, June 2008, p. 2420-2427, Vol. 76, No. 6
0019-9567/08/$08.00+0     doi:10.1128/IAI.00156-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



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