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Infection and Immunity, June 2008, p. 2642-2650, Vol. 76, No. 6
0019-9567/08/$08.00+0     doi:10.1128/IAI.01639-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

In LipL32, the Major Leptospiral Lipoprotein, the C Terminus Is the Primary Immunogenic Domain and Mediates Interaction with Collagen IV and Plasma Fibronectin {triangledown}

Pricila Hauk,1,2 Felipe Macedo,1 Eliete Caló Romero,3 Sílvio Arruda Vasconcellos,4 Zenaide Maria de Morais,4 Angela Silva Barbosa,1,5* and Paulo Lee Ho1,2*

Centro de Biotecnologia, Instituto Butantan, 05503-900 São Paulo, SP, Brazil,1 Programa de Pós-Graduação Interunidades em Biotecnologia USP/IPT/Instituto Butantan, Instituto de Ciências Biomédicas da USP, 05508-900 São Paulo, SP, Brazil,2 Seção de Bacteriologia, Instituto Adolfo Lutz, 01246-902 São Paulo, SP, Brazil,3 Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, 05508-270 São Paulo, SP, Brazil,4 Laboratório de Bacteriologia, Instituto Butantan, 05503-900 São Paulo, SP, Brazil5

Received 10 December 2007/ Returned for modification 27 January 2008/ Accepted 26 March 2008

LipL32 is the major leptospiral outer membrane lipoprotein expressed during infection and is the immunodominant antigen recognized during the humoral immune response to leptospirosis in humans. In this study, we investigated novel aspects of LipL32. In order to define the immunodominant domains(s) of the molecule, subfragments corresponding to the N-terminal, intermediate, and C-terminal portions of the LipL32 gene were cloned and the proteins were expressed and purified by metal affinity chromatography. Our immunoblot results indicate that the C-terminal and intermediate domains of LipL32 are recognized by sera of patients with laboratory-confirmed leptospirosis. An immunoglobulin M response was detected exclusively against the LipL32 C-terminal fragment in both the acute and convalescent phases of illness. We also evaluated the capacity of LipL32 to interact with extracellular matrix (ECM) components. Dose-dependent, specific binding of LipL32 to collagen type IV and plasma fibronectin was observed, and the binding capacity could be attributed to the C-terminal portion of this molecule. Both heparin and gelatin could inhibit LipL32 binding to fibronectin in a concentration-dependent manner, indicating that the 30-kDa heparin-binding and 45-kDa gelatin-binding domains of fibronectin are involved in this interaction. Taken together, our results provide evidence that the LipL32 C terminus is recognized early in the course of infection and is the domain responsible for mediating interaction with ECM proteins.

* Corresponding author. Mailing address for Angela Silva Barbosa: Centro de Biotecnologia/Laboratório de Bacteriologia, Instituto Butantan, 05503-900 São Paulo, SP, Brazil. Phone: 55-11-3726-7222, ext. 2261. Fax: 55-11-3726-1505. E-mail: angelabarbosa{at}butantan.gov.br. Mailing address for Paulo Lee Ho: Centro de Biotecnologia, Instituto Butantan, 05503-900 São Paulo, SP, Brazil. Phone: 55-11-3726-7222, ext. 2244. Fax: 55-11-3726-1505. E-mail: hoplee{at}butantan.gov.br

{triangledown} Published ahead of print on 7 April 2008.

Editor: A. Camilli

Infection and Immunity, June 2008, p. 2642-2650, Vol. 76, No. 6
0019-9567/08/$08.00+0     doi:10.1128/IAI.01639-07
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



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