This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Chakravarty, S.
Right arrow Articles by Zavala, F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Chakravarty, S.
Right arrow Articles by Zavala, F.

 Previous Article  |  Next Article 

Infection and Immunity, August 2008, p. 3628-3631, Vol. 76, No. 8
0019-9567/08/$08.00+0     doi:10.1128/IAI.00471-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.

Effector CD8+ T Lymphocytes against Liver Stages of Plasmodium yoelii Do Not Require Gamma Interferon for Antiparasite Activity{triangledown}

Sumana Chakravarty,{dagger} G. Christian Baldeviano, Michael G. Overstreet, and Fidel Zavala*

Department of Molecular Microbiology and Immunology, Malaria Research Institute, Bloomberg School of Public Health, Johns Hopkins University, 615 N. Wolfe Street, Baltimore, Maryland 21205

Received 15 April 2008/ Returned for modification 14 May 2008/ Accepted 22 May 2008

The protective immune response against liver stages of the malaria parasite critically requires CD8+ T cells. Although the nature of the effector mechanism utilized by these cells to repress parasite development remains unclear, a critical role for gamma interferon (IFN-{gamma}) has been widely assumed based on circumstantial evidence. However, the requirement for CD8+ T-cell-mediated IFN-{gamma} production in protective immunity to this pathogen has not been directly tested. In this report, we use an adoptive transfer strategy with circumsporozoite (CS) protein-specific transgenic T cells to examine the role of CD8+ T-cell-derived IFN-{gamma} production in Plasmodium yoelii-infected mice. We show that despite a marginal reduction in the expansion of naive IFN-{gamma}-deficient CS-specific transgenic T cells, their antiparasite activity remains intact. Further, adoptively transferred IFN-{gamma}-deficient CD8+ T cells were as efficient as their wild-type counterparts in limiting parasite growth in naive mice. Taken together, these studies demonstrate that IFN-{gamma} secretion by CS-specific CD8+ T cells is not essential to protect mice against live sporozoite challenge.

* Corresponding author. Mailing address: Department of Molecular Microbiology and Immunology, Malaria Research Institute, Bloomberg School of Public Health, Johns Hopkins University, 615 N. Wolfe Street, Baltimore, MD 21205. Phone: (443) 287-1769. Fax: (410) 955-0105. E-mail: fzavala{at}jhsph.edu

{triangledown} Published ahead of print on 2 June 2008.

Editor: J. L. Flynn

{dagger} Present address: Sanaria, Inc., 9800 Medical Center Drive, Rockville, MD 20850.

Infection and Immunity, August 2008, p. 3628-3631, Vol. 76, No. 8
0019-9567/08/$08.00+0     doi:10.1128/IAI.00471-08
Copyright © 2008, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Trimnell, A., Takagi, A., Gupta, M., Richie, T. L., Kappe, S. H., Wang, R. (2009). Genetically Attenuated Parasite Vaccines Induce Contact-Dependent CD8+ T Cell Killing of Plasmodium yoelii Liver Stage-Infected Hepatocytes. J. Immunol. 183: 5870-5878 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»