This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental material
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Google Scholar
Right arrow Articles by Nicholson, T. L.
Right arrow Articles by Brockmeier, S. L.
PubMed
Right arrow PubMed Citation
Right arrow Articles by Nicholson, T. L.
Right arrow Articles by Brockmeier, S. L.

 Previous Article  |  Next Article 

Infection and Immunity, October 2009, p. 4221-4231, Vol. 77, No. 10
0019-9567/09/$08.00+0     doi:10.1128/IAI.00136-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Microarray and Functional Analysis of Growth Phase-Dependent Gene Regulation in Bordetella bronchiseptica{triangledown} ,{dagger}

Tracy L. Nicholson,1* Anne M. Buboltz,2,3 Eric T. Harvill,2 and Susan L. Brockmeier1

Respiratory Diseases of Livestock Research Unit, National Animal Disease Center, Agricultural Research Service, USDA, Ames, Iowa,1 Department of Veterinary and Biomedical Sciences, The Pennsylvania State University, University Park, Pennsylvania,2 Graduate Program in Biochemistry, Microbiology and Molecular Biology, The Pennsylvania State University, University Park, Pennsylvania3

Received 3 February 2009/ Returned for modification 10 March 2009/ Accepted 30 July 2009

Growth phase-dependent gene regulation has recently been demonstrated to occur in Bordetella pertussis, with many transcripts, including known virulence factors, significantly decreasing during the transition from logarithmic to stationary-phase growth. Given that B. pertussis is thought to have derived from a Bordetella bronchiseptica-like ancestor, we hypothesized that growth phase-dependent gene regulation would also occur in B. bronchiseptica. Microarray analysis revealed and quantitative real-time PCR (qRT-PCR) confirmed that growth phase-dependent gene regulation occurs in B. bronchiseptica, resulting in prominent temporal shifts in global gene expression. Two virulence phenotypes associated with these gene expression changes were tested. We found that growth-dependent increases in expression of some type III secretion system (TTSS) genes led to a growth phase-dependent increase in a TTSS-dependent function, cytotoxicity. Although the transcription of genes encoding adhesins previously shown to mediate adherence was decreased in late-log and stationary phases, we found that the adherence of B. bronchiseptica did not decrease in these later phases of growth. Microarray analysis revealed and qRT-PCR confirmed that growth phase-dependent gene regulation occurred in both Bvg+ and Bvg– phase-locked mutants, indicating that growth phase-dependent gene regulation in B. bronchiseptica can function independently from the BvgAS regulatory system.

* Corresponding author. Mailing address: Respiratory Diseases of Livestock Research Unit, National Animal Disease Center, ARS, USDA, 2300 Dayton Ave., Ames, IA 50010. Phone: (515) 663-7349. Fax: (515) 663-7458. E-mail: tracy.nicholson{at}ARS.USDA.GOV

{triangledown} Published ahead of print on 10 August 2009.

{dagger} Supplemental material for this article may be found at http://iai.asm.org/.

Editor: A. Camilli

Infection and Immunity, October 2009, p. 4221-4231, Vol. 77, No. 10
0019-9567/09/$08.00+0     doi:10.1128/IAI.00136-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»