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Infection and Immunity, October 2009, p. 4510-4517, Vol. 77, No. 10
0019-9567/09/$08.00+0     doi:10.1128/IAI.00360-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Inhibitory Antibodies Specific for the 19-Kilodalton Fragment of Merozoite Surface Protein 1 Do Not Correlate with Delayed Appearance of Infection with Plasmodium falciparum in Semi-Immune Individuals in Vietnam {triangledown}

E. Elsa Herdiana Murhandarwati,1,{dagger} Lina Wang,1 Casilda G. Black,1 Doan Hanh Nhan,2 Thomas L. Richie,3 and Ross L. Coppel1*

Department of Microbiology, Monash University, Clayton, Victoria 3800, Australia,1 Institute for Malariology, Parasitology and Entomology, Hanoi, Vietnam,2 U.S. Military Malaria Vaccine Program, Naval Medical Research Centre/Walter Reed Army Institute of Research, Silver Spring, Maryland 209103

Received 29 March 2009/ Returned for modification 4 May 2009/ Accepted 13 July 2009

Inhibitory antibodies specific for the 19-kDa fragment of merozoite surface protein 1 (MSP119) are a significant component of inhibitory responses in individuals immune to malaria. Nevertheless, conflicting results have been obtained in determining whether this antibody specificity correlates with protection in residents of areas where malaria is endemic. In this study, we examined sera collected from a population of semi-immune individuals living in an area of Vietnam with meso-endemicity during a 6-month period. We used two Plasmodium falciparum parasite lines that express either endogenous MSP119 or the homologous region from Plasmodium yoelii to measure the MSP119-specific inhibitory activity. We showed that (i) the level of MSP119-specific inhibitory antibodies was not associated with a delay in P. falciparum infection, (ii) MSP119-specific inhibitory antibodies declined significantly during the convalescent period after infection, and (iii) there was no significant correlation between the MSP119-specific inhibitory antibodies and the total antibodies measured by enzyme-linked immunosorbent assay. These results have implications for understanding naturally acquired immunity to malaria and for the development and evaluation of MSP119-based vaccines.

* Corresponding author. Mailing address: Department of Microbiology, Monash University, Clayton, Victoria 3800, Australia. Phone: (61) 3 9902 9147. Fax: (61) 3 9902 9222. E-mail: ross.coppel{at}med.monash.edu.au

{triangledown} Published ahead of print on 20 July 2009.

Editor: J. F. Urban, Jr.

{dagger} Present address: Department of Parasitology, Faculty of Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia.

Infection and Immunity, October 2009, p. 4510-4517, Vol. 77, No. 10
0019-9567/09/$08.00+0     doi:10.1128/IAI.00360-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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