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Infection and Immunity, November 2009, p. 4877-4886, Vol. 77, No. 11
0019-9567/09/$08.00+0     doi:10.1128/IAI.00698-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Genome-Wide Study of Pseudomonas aeruginosa Outer Membrane Protein Immunogenicity Using Self-Assembling Protein Microarrays{triangledown} ,{dagger}

Wagner R. Montor,1,{ddagger} Jin Huang,2 Yanhui Hu,1 Eugenie Hainsworth,1 Susan Lynch,3 Jeannine-Weiner Kronish,3 Claudia L. Ordonez,4 Tanya Logvinenko,5 Stephen Lory,2 and Joshua LaBaer1*

Harvard Institute of Proteomics, Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, Massachusetts 02115,1 Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115,2 Department of Anesthesia and Perioperative Care, University of California—San Francisco, San Francisco, California 94143,3 Boston Children's Hospital, Boston, Massachusetts 02115,4 Institute for Clinical Research and Health Policy Studies, Tufts New England Medical Center, Boston, Massachusetts 021105

Received 19 June 2009/ Returned for modification 22 July 2009/ Accepted 2 September 2009

Pseudomonas aeruginosa is responsible for potentially life-threatening infections in individuals with compromised defense mechanisms and those with cystic fibrosis. P. aeruginosa infection is notable for the appearance of a humoral response to some known antigens, such as flagellin C, elastase, alkaline protease, and others. Although a number of immunogenic proteins are known, no effective vaccine has been approved yet. Here, we report a comprehensive study of all 262 outer membrane and exported P. aeruginosa PAO1 proteins by a modified protein microarray methodology called the nucleic acid-programmable protein array. From this study, it was possible to identify 12 proteins that trigger an adaptive immune response in cystic fibrosis and acutely infected patients, providing valuable information about which bacterial proteins are actually recognized by the immune system in vivo during the natural course of infection. The differential detections of these proteins in patients and controls proved to be statistically significant (P < 0.01). The study provides a list of potential candidates for the improvement of serological diagnostics and the development of vaccines.

* Corresponding author. Present address: Virginia G. Piper Center for Personalized Diagnostics, Biodesign Institute, Arizona State University, Tempe, AZ 85287. Phone: (480) 965-2805. Fax: (480) 965-3051. E-mail: jlabaer{at}asu.edu

{triangledown} Published ahead of print on 8 September 2009.

{dagger} Supplemental material for this article may be found at http://iai.asm.org/.

Editor: J. N. Weiser

{ddagger} Present address: Department of Physiology, Santa Casa de São Paulo Medical School, São Paulo, Brazil.

Infection and Immunity, November 2009, p. 4877-4886, Vol. 77, No. 11
0019-9567/09/$08.00+0     doi:10.1128/IAI.00698-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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