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Infection and Immunity, December 2009, p. 5543-5550, Vol. 77, No. 12
0019-9567/09/$08.00+0     doi:10.1128/IAI.00532-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Partial Protection against Helicobacter pylori in the Absence of Mast Cells in Mice{triangledown}

Hua Ding,1 John G. Nedrud,2 Barry Wershil,3 Raymond W. Redline,2 Thomas G. Blanchard,1 and Steven J. Czinn1*

Department of Pediatrics, University of Maryland School of Medicine, Baltimore, Maryland,1 Department of Pathology, Case Western Reserve University, School of Medicine, Cleveland, Ohio,2 Department of Pediatrics, Northwestern University Feinberg School of Medicine, Chicago, Illinois3

Received 13 May 2009/ Returned for modification 5 September 2009/ Accepted 30 September 2009

The goal of this study is to evaluate the contribution of mast cells to Helicobacter pylori immunity in a model of vaccine-induced protection. Mast cell-deficient KitlSl/KitlSl-d and control mice were immunized with H. pylori sonicate plus cholera toxin and challenged with H. pylori, and the bacterial loads, inflammatory infiltrates, and cytokine responses were evaluated and compared at 1, 2, and 4 weeks postchallenge. In vitro stimulation assays were performed using bone marrow-derived mast cells, and recall assays were performed with spleen cells of immunized mast cell-deficient and wild-type mice. Bacterial clearance was observed by 2 weeks postchallenge in mast cell-deficient mice. The bacterial load was reduced by 4.0 log CFU in wild-type mice and by 1.5 log CFU in mast cell-deficient mice. Neutrophil numbers in the gastric mucosa of immune KitlSl/KitlSl-d mice were lower than those for immune wild-type mice (P < 0.05). Levels of gastric interleukin-17 (IL-17) and tumor necrosis factor alpha (TNF-{alpha}) were also significantly lower in immune KitlSl/KitlSl-d mice than in wild-type mice (P < 0.001). Immunized mast cell-deficient and wild-type mouse spleen cells produced IFN-{gamma} and IL-17 in response to H. pylori antigen stimulation. TNF-{alpha} and CXC chemokines were detected in mast cell supernatants after 24 h of stimulation with H. pylori antigen. The results indicate that mast cells are not essential for but do contribute to vaccine-induced immunity and that mast cells contribute to neutrophil recruitment and inflammation in response to H. pylori.

* Corresponding author. Mailing address: Department of Pediatrics, University of Maryland School of Medicine, 22 South Greene Street, Room N5E17, Baltimore, MD 21201. Phone: (410) 328-6777. Fax: (410) 328-8742. E-mail: sczinn{at}peds.umaryland.edu

{triangledown} Published ahead of print on 12 October 2009.

Editor: S. R. Blanke

Infection and Immunity, December 2009, p. 5543-5550, Vol. 77, No. 12
0019-9567/09/$08.00+0     doi:10.1128/IAI.00532-09
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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