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Infection and Immunity, March 2009, p. 1182-1188, Vol. 77, No. 3
0019-9567/09/$08.00+0     doi:10.1128/IAI.01150-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Role of Neutrophils in Response to Bordetella pertussis Infection in Mice{triangledown}

Charlotte Andreasen{dagger} and Nicholas H. Carbonetti*

Department of Microbiology and Immunology, University of Maryland School of Medicine, Baltimore, Maryland

Received 15 September 2008/ Returned for modification 28 October 2008/ Accepted 16 December 2008

Pertussis is an acute respiratory disease caused by the bacterium Bordetella pertussis, for which humans are the only known reservoir. During infection, B. pertussis releases several toxins, including pertussis toxin (PT) and adenylate cyclase toxin (ACT), which have both been shown to play roles in promoting bacterial growth during early infection in a mouse model. Furthermore, in vitro and in vivo studies suggest that PT and ACT affect neutrophil chemotaxis and/or function, thereby altering the innate immune response. In this study we depleted animals of neutrophils to investigate whether neutrophils play a protective role during B. pertussis infection in mice. In addition, by infection with toxin-deficient strains, we investigated whether neutrophils are the main targets for PT and/or ACT activity in promoting bacterial growth. Surprisingly, we found no role for neutrophils during B. pertussis infection in naïve mice. However, in previously infected (immune) mice or in mice receiving immune serum, we observed a significant role for neutrophils during infection. Furthermore, in this immune mouse model our evidence indicates that neutrophils appear to be the main target cells for ACT, but not for PT.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, University of Maryland School of Medicine, 660 W. Redwood St., HH 324, Baltimore, MD 21201. Phone: (410) 706-7677. Fax: (410) 706-2129. E-mail: ncarbone{at}umaryland.edu

{triangledown} Published ahead of print on 22 December 2008.

Editor: A. J. Bäumler

{dagger} Present address: Section of Pulmonary and Critical Care Medicine, Yale University School of Medicine, New Haven, CT.

Infection and Immunity, March 2009, p. 1182-1188, Vol. 77, No. 3
0019-9567/09/$08.00+0     doi:10.1128/IAI.01150-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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