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Infection and Immunity, March 2009, p. 1216-1221, Vol. 77, No. 3
0019-9567/09/$08.00+0     doi:10.1128/IAI.01109-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Effect of Chlamydiaphage {varphi}CPG1 on the Course of Conjunctival Infection with "Chlamydia caviae" in Guinea Pigs{triangledown}

Roger G. Rank,1* Anne K. Bowlin,1 Stefania Cané,1 Huizhong Shou,3 Zhi Liu,3 Uma M. Nagarajan,2 and Patrik M. Bavoil3

Departments of Microbiology and Immunology,1 Pediatrics, University of Arkansas for Medical Sciences and Arkansas Children's Hospital Research Institute, Little Rock, Arkansas,2 Department of Microbial Pathogenesis, University of Maryland Dental School, Baltimore, Maryland3

Received 5 September 2008/ Returned for modification 25 October 2008/ Accepted 4 January 2009

Over the last several years, four different phages of chlamydiae, in addition to a phage associated with Chlamydia psittaci isolated from an ornithosis infection in ducks over 25 years ago, have been described and characterized. While these phages and their chlamydial host specificities have been characterized in vitro, there is virtually nothing known about the interaction of the phage with chlamydiae in their natural animal host. {varphi}CPG1 is a lytic phage specific for "Chlamydia caviae," which is a natural parasite of the guinea pig. In this study, guinea pigs were inoculated in the conjunctiva with suspensions of {varphi}CPG1 and C. caviae and the effect on the development of pathology and on the course of chlamydial infection in the conjunctiva was determined. The presence of phage delayed the appearance of the peak level of chlamydiae in the animal and decreased the pathological response. Evidence for replication of the phage in C. caviae in the conjunctival tissue was observed. Modifying the ratio of phage to chlamydiae altered the course of infection and affected phage replication. There was no evidence for the phage increasing the virulence of C. caviae infection.

* Corresponding author. Mailing address: Arkansas Children's Hospital Research Institute, 1120 Marshall Street, Little Rock, AR 72202. Phone: (501) 364-2474. Fax: (501) 364-2403. E-mail: rankrogerg{at}uams.edu

{triangledown} Published ahead of print on 12 January 2009.

Editor: S. R. Blanke

Infection and Immunity, March 2009, p. 1216-1221, Vol. 77, No. 3
0019-9567/09/$08.00+0     doi:10.1128/IAI.01109-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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