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Infection and Immunity, April 2009, p. 1285-1292, Vol. 77, No. 4
0019-9567/09/$08.00+0     doi:10.1128/IAI.01062-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Host Complement Regulatory Protein CD59 Is Transported to the Chlamydial Inclusion by a Golgi Apparatus-Independent Pathway {triangledown}

Ayako Hasegawa,1,{dagger} L. Farah Sogo,1,{dagger} Ming Tan,2 and Christine Sütterlin1*

Department of Developmental and Cell Biology,1 Departments of Microbiology & Molecular Genetics and Medicine, University of California, Irvine, Irvine, California 92697-23002

Received 26 August 2008/ Returned for modification 30 September 2008/ Accepted 13 January 2009

Chlamydia is an obligate intracellular bacterium that grows and replicates inside a cytoplasmic inclusion. We report that a host protein, CD59, which regulates complement function at the surfaces of uninfected cells, can be detected at the membrane of the chlamydial inclusion. This localization to the inclusion membrane was specific for CD59 and not a general feature of other glycosylphosphatidylinositol (GPI)-anchored proteins or representative cell surface proteins. Using differential permeabilization studies, we showed that CD59 is localized to the luminal but not the cytoplasmic face of the inclusion membrane, consistent with membrane association via its GPI anchor. Furthermore, CD59 was present at the inclusion even when we prevented it from associating with membrane microdomains via the GPI anchor or when we inhibited general protein transport to the cell surface, indicating that a conventional Golgi apparatus-dependent trafficking mechanism was not involved. Based on these findings, we propose that selected host proteins are trafficked to the inclusion by a Golgi apparatus-independent pathway during a Chlamydia infection.

* Corresponding author. Mailing address: Department of Developmental and Cell Biology, University of California, Irvine, 3128 Natural Sciences I, Irvine, CA 92697-2300. Phone: (949) 824-7140. Fax: (949) 824-4709. E-mail: suetterc{at}uci.edu

{triangledown} Published ahead of print on 21 January 2009.

Editor: R. P. Morrison

{dagger} A.H. and L.F.S. contributed equally to this work.

Infection and Immunity, April 2009, p. 1285-1292, Vol. 77, No. 4
0019-9567/09/$08.00+0     doi:10.1128/IAI.01062-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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