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Infection and Immunity, April 2009, p. 1514-1523, Vol. 77, No. 4
0019-9567/09/$08.00+0     doi:10.1128/IAI.01739-07
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Vaccination Route That Induces Transforming Growth Factor β Production Fails To Elicit Protective Immunity against Leishmania donovani Infection

Sudipta Bhowmick, Tuhina Mazumdar, and Nahid Ali^*

Infectious Diseases and Immunology Division, Indian Institute of Chemical Biology, 4 Raja S. C. Mullick Road, Jadavpur, Kolkata 700032, India

Received 28 December 2007/ Returned for modification 13 February 2008/ Accepted 13 January 2009

BALB/c mice immunized intraperitoneally (i.p.) and intravenously (i.v.) with Leishmania donovani promastigote membrane antigens (LAg), either free or encapsulated in liposomes, were protected against challenge infection with L. donovani, whereas mice immunized by the subcutaneous (s.c.) and intramuscular routes were not protected. Protected mice showed strong parasite resistance in both the liver and spleen, along with enhanced immunoglobulin G2a and delayed-type hypersensitivity responses. Again, mice vaccinated through the i.p. and i.v. routes showed high levels of NO production after challenge infection. s.c. vaccination resulted in an increased capacity of the spleen cells to produce prechallenge transforming growth factor β (TGF-β) levels during the in vitro antigen recall response, whereas i.p. immunization induced production of prechallenge gamma interferon, interleukin-12 (IL-12), and IL-4 levels, with a Th1 bias. Exposure to antigen-stimulated splenocyte supernatants of i.p. but not s.c. immunized mice activated macrophages for in vitro parasite killing. As an enhanced level of TGF-β was detected in supernatants from unprotected s.c. immunized mice, neutralization by anti-TGF-β antibody enhanced in vitro macrophage killing activity. The suppressive role of this cytokine was evaluated in vivo by vaccination with liposomal LAg and anti-TGF-β antibody. Upon parasite challenge, these animals showed significant protection in both the liver and spleen. Moreover, the addition of recombinant TGF-β in splenocyte supernatants of i.p. immunized mice in vitro as well as in vivo inhibited the protective ability of the macrophages by the i.p. route. Thus, the induction of high prechallenge TGF-β limits the efficacy of vaccination by routes that are nonprotective.

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* Corresponding author. Mailing address: Infectious Diseases and Immunology Division, Indian Institute of Chemical Biology, 4 Raja S. C. Mullick Road, Jadavpur, Kolkata 700032, India. Phone: 91-33-2473-3491. Fax: 91-33-2473-0284. E-mail: nali{at}iicb.res.in

Published ahead of print on 21 January 2009.

Editor: J. F. Urban, Jr.

Present address: Pulmonary Division, Department of Medicine, Baylor College of Medicine, Houston, TX 77030.

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Infection and Immunity, April 2009, p. 1514-1523, Vol. 77, No. 4
0019-9567/09/$08.00+0     doi:10.1128/IAI.01739-07
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

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