This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Moens, L.
Right arrow Articles by Bossuyt, X.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Moens, L.
Right arrow Articles by Bossuyt, X.

 Previous Article  |  Next Article 

Infection and Immunity, May 2009, p. 1976-1980, Vol. 77, No. 5
0019-9567/09/$08.00+0     doi:10.1128/IAI.01091-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Generation of Antibody Responses to Pneumococcal Capsular Polysaccharides Is Independent of CD1 Expression in Mice{triangledown}

Leen Moens,1,{dagger} Axel Jeurissen,1,{dagger} Stefan Nierkens,2 Louis Boon,3 Luc Van Kaer,4 Ahmad Kasran,5 Greet Wuyts,1 Jan L. Ceuppens,5 and Xavier Bossuyt1*

Experimental Laboratory Medicine, Department of Medical Diagnostic Sciences, Faculty of Medicine, Catholic University Leuven, Leuven, Belgium,1 Department of Tumor Immunology, Nijmegen Centre for Molecular Life Sciences, University Medical Centre Nijmegen, The Netherlands,2 Bioceros B.V., Utrecht, The Netherlands,3 Laboratory of Experimental Immunology, Department of Pathophysiology, Faculty of Medicine, Catholic University Leuven, Leuven, Belgium,5 Department of Microbiology and Immunology, Vanderbilt University School of Medicine, Nashville, Tennessee4

Received 2 September 2008/ Returned for modification 23 October 2008/ Accepted 27 January 2009

Streptococcus pneumoniae is a bacterial microorganism that frequently causes serious infection, particularly in children and the elderly. Protection against infection with S. pneumoniae is based mainly on the generation of antibodies to the pneumococcal capsular polysaccharides (caps-PS), but the mechanisms responsible for the generation of anticapsular antibodies remain incompletely understood. The aim of the present study was to evaluate the role of CD1-restricted T cells in the antibody response to caps-PS. When immunized with Pneumo23, wild-type mice and CD1 knockout mice on BALB/c and C57BL/6 backgrounds generated immunoglobulin M (IgM) and IgG antibody responses to soluble caps-PS that were comparable. Similar results were obtained after immunization with heat-inactivated S. pneumoniae. The IgM and IgG antibody response of wild-type mice to Pneumo23 was not affected by an antagonizing monoclonal anti-CD1 antibody treatment. In summary, our data provide evidence that the antibody response to caps-PS is generated independently of CD1 expression.

* Corresponding author. Mailing address: University Hospital Leuven, Laboratory Medicine/Immunology, Campus Gasthuisberg, CDG 7th Floor, Herestraat 49, B-3000 Leuven, Belgium. Phone: 32 16 34 70 09. Fax: 32 16 34 79 31. E-mail: Xavier.bossuyt{at}uz.kuleuven.ac.be

{triangledown} Published ahead of print on 2 February 2009.

Editor: V. J. DiRita

{dagger} These authors equally contributed to the study.

Infection and Immunity, May 2009, p. 1976-1980, Vol. 77, No. 5
0019-9567/09/$08.00+0     doi:10.1128/IAI.01091-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»