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Infection and Immunity, May 2009, p. 2043-2050, Vol. 77, No. 5
0019-9567/09/$08.00+0     doi:10.1128/IAI.01388-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

Staphylococcus aureus Superantigens Elicit Redundant and Extensive Human Vβ Patterns {triangledown}

Damien Thomas,1 Olivier Dauwalder,1 Virginie Brun,2 Cedric Badiou,1 Tristan Ferry,1 Jerome Etienne,1 François Vandenesch,1 and Gerard Lina1*

Université Lyon 1, Centre National de Référence des Staphylocoques, INSERM U851, IFR128 Laennec, 7 Rue Guillaume Paradin, 69372 Lyon Cedex 08, France,1 Laboratoire d'Etude de la Dynamique des Protéomes, U880 CEA/DSV/iRTSV/INSERM/UJF, 17 Rue des Martyrs, 38054 Grenoble Cedex, France2

Received 13 November 2008/ Returned for modification 17 November 2008/ Accepted 23 February 2009

Staphylococcus aureus can produce a wide variety of exotoxins, including toxic shock syndrome toxin 1 (TSST-1), staphylococcal enterotoxins, and staphylococcal enterotoxin-like toxins. These toxins share superantigenic activity. To investigate the β chain (Vβ) specificities of each of these toxins, TSST-1 and all known S. aureus enterotoxins and enterotoxin-like toxins were produced as recombinant proteins and tested for their ability to induce the selective in vitro expansion of human T cells bearing particular Vβ T-cell receptors (TCR). Although redundancies were observed between the toxins and the Vβ populations, each toxin induced the expansion of distinct Vβ subsets, including enterotoxin H and enterotoxin-like toxin J. Surprisingly, the Vβ signatures were not associated with a specific phylogenic group of toxins. Interestingly, each human Vβ analyzed in this study was stimulated by at least one staphylococcal superantigen, suggesting that the bacterium derives a selective advantage from targeting the entire human TCR Vβ panel.

* Corresponding author. Mailing address: Université Lyon 1, Centre National de Référence des Staphylocoques, INSERM U851, IFR128 Laennec, 7 Rue Guillaume Paradin, 69372 Lyon Cedex 08, France. Phone: 33 478 77 86 57. Fax: 33 478 77 86 58. E-mail: gerard.lina{at}chu-lyon.fr

{triangledown} Published ahead of print on 2 March 2009.

Editor: S. R. Blanke

Infection and Immunity, May 2009, p. 2043-2050, Vol. 77, No. 5
0019-9567/09/$08.00+0     doi:10.1128/IAI.01388-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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