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Infection and Immunity, June 2009, p. 2568-2575, Vol. 77, No. 6
0019-9567/09/$08.00+0     doi:10.1128/IAI.01537-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.

O-Antigen-Negative Salmonella enterica Serovar Typhimurium Is Attenuated in Intestinal Colonization but Elicits Colitis in Streptomycin-Treated Mice{triangledown}

Karin Ilg,{dagger} Kathrin Endt,{dagger} Benjamin Misselwitz, Bärbel Stecher, Markus Aebi,* and Wolf-Dietrich Hardt*

Institut für Mikrobiologie, Eidgenössische Technische Hochschule, ETH Zürich, Wolfgang-Pauli-Str. 10, CH-8093 Zürich, Switzerland

Received 18 December 2008/ Returned for modification 3 February 2009/ Accepted 30 March 2009

Lipopolysaccharide (LPS) is a major constituent of the outer membrane and an important virulence factor of Salmonella enterica subspecies 1 serovar Typhimurium (serovar Typhimurium). To evaluate the role of LPS in eliciting intestinal inflammation in streptomycin-treated mice, we constructed an O-antigen-deficient serovar Typhimurium strain through deletion of the wbaP gene. The resulting strain was highly susceptible to human complement activity and the antimicrobial peptide mimic polymyxin B. Furthermore, it showed a severe defect in motility and an attenuated phenotype in a competitive mouse infection experiment, where the {Delta}wbaP strain (SKI12) was directly compared to wild-type Salmonella. Nevertheless, the {Delta}wbaP strain (SKI12) efficiently invaded HeLa cells in vitro and elicited acute intestinal inflammation in streptomycin-pretreated mice. Our experiments prove that the presence of complete LPS is not essential for in vitro invasion or for triggering acute colitis.

* Corresponding author. Mailing address for Markus Aebi: Institut für Mikrobiologie, ETH Zürich, Departement Biologie, ETH Hönggerberg, HCI F 407, Wolfgang-Pauli Str. 10, CH-8093 Zürich, Switzerland. Phone: 41 44 632 64 13. Fax: 41 44 632 11 48. E-mail: aebi{at}micro.biol.ethz.ch. Mailing address for Wolf-Dietrich Hardt: Institut für Mikrobiologie, ETH Zürich, Departement Biologie, ETH Hönggerberg, HCI G 417, Wolfgang-Pauli Str. 10, CH-8093 Zürich, Switzerland. Phone: 41 44 632 51 43. Fax: 41 44 632 11 29. E-mail: hardt{at}micro.biol.ethz.ch

{triangledown} Published ahead of print on 13 April 2009.

Editor: A. J. Bäumler

{dagger} Both authors contributed equally to this work.

Infection and Immunity, June 2009, p. 2568-2575, Vol. 77, No. 6
0019-9567/09/$08.00+0     doi:10.1128/IAI.01537-08
Copyright © 2009, American Society for Microbiology. All Rights Reserved.





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